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Research Journal of Microbiology
  Year: 2008 | Volume: 3 | Issue: 8 | Page No.: 559-564
DOI: 10.3923/jm.2008.559.564
Isolation of Cold Tolerant Antifungal Strains of Trichoderma sp. From Glacial Sites of Indian Himalayan Region
A. Ghildiyal and A. Pandey

Abstract:
Three species of Trichoderma viz., T. harzianum, T. konengii and T. viride have been isolated from the soil samples collected from forest sites in higher altitudes of Indian Himalayan Region. The species could grow between 9 to 35°C temperature and 4 to 12 pH on agar plates; the optimum requirement being 24°C and 5.5 pH, respectively. Further incubation of the agar plates showing normal growth of Trichoderma sp. at 4°C, induced heavy sporulation in three weeks of time. Induction of sporulation on exposure to low temperature appeared to be a strategy for survival of these species in extreme cold environment experiencing sub zero temperatures. Antifungal activities were demonstrated between Trichoderma sp. and phytopathogenic fungi in dual cultures. The antifungal metabolites produced by Trichoderma sp., diffusible as well as volatile, caused abnormalities in fungal structures of pathogenic fungi. Plant growth promotion abilities of Trichoderma sp. was also demonstrated through a plant based bioassay in greenhouse. The study is important for documentation of microbial diversity of Indian Himalayan Region (IHR) and determination of the associated biotechnological applications.
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How to cite this article:

A. Ghildiyal and A. Pandey, 2008. Isolation of Cold Tolerant Antifungal Strains of Trichoderma sp. From Glacial Sites of Indian Himalayan Region. Research Journal of Microbiology, 3: 559-564.

DOI: 10.3923/jm.2008.559.564

URL: https://scialert.net/abstract/?doi=jm.2008.559.564

 
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