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Research Journal of Microbiology
  Year: 2008 | Volume: 3 | Issue: 3 | Page No.: 122-128
DOI: 10.3923/jm.2008.122.128
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Phenazine Pigments from Pseudomonas aeruginosa and Their Application as Antibacterial Agent and Food Colourants
Samanta Saha, R. Thavasi and S. Jayalakshmi

This study deals with isolation and identification of Pseudomonas aeruginosa from marine environment for phenazine pigment production. Pigment production results revealed that, the strain could able to produce two different pigments namely, pyocyanin and pyorubrin. Maximum biomass was observed at 66 h of incubation, but the pigment production seems to be continuous throughout the culture period (72 h). Antibacterial activity of the pigments was evaluated against pathogenic bacteria, maximum growth inhibitory activity was observed with pyocyanin and pyorubrin at 20 μL concentration (1.7 and 1.3 cm, respectively) against Citrobacter sp. Hemolytic activity of the pigments inferred that, hemolysis was observed with both pigments at 15, 20 and 25 μL concentration and no hemolysis was found at 5, 10 and 15 μL. The pigments were evaluated for their potential as food colourants with agar. Pleasant colouration was observed with pyocyanin and pyorubrin at 25 mg mLG-1 concentration.
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  •    Contribution to the Ecobiological Study of the Pseudomonas fluorescens Rhizobacteria
  •    Hemolytic Activities from Ascidian Polyclinum madrasensis Sebestian, 1952 and Phallusia nigra Savigny, 1816 from Tuticorin Coast of India
How to cite this article:

Samanta Saha, R. Thavasi and S. Jayalakshmi, 2008. Phenazine Pigments from Pseudomonas aeruginosa and Their Application as Antibacterial Agent and Food Colourants. Research Journal of Microbiology, 3: 122-128.

DOI: 10.3923/jm.2008.122.128


08 February, 2011
amany salama el sharkawy:
dear sir,
I'm a master student from egypt,biotechnology department,institute of graduate studies and research,alexandria,i succeeded to isolate marine pseudomonas aeruginosa producing pyocianin and pyorubrin,but i used pigment production media at 28 degree temprature a nd 48 hour incubation,i wish to know if you obtained the blue green pyocyanin at the same cultural conditions as to obtain the brown red pyorubrin???it was different in my case,,on the other hand ,,did you try acetone precipitation and coloum chromatography to purify the product??if you are willing to share information and discuss it with me please e mail .best of luck in your research.thanks,bye
01 March, 2011
sukhendu mandal:

Dear amany in case of pseudomonas there are number of other pigments produced. so you cant say that it is pyorubin and the other case is pyocyanin turns red in acidic condition at pH< 4.9. you need to first extract bothn in crude form then you can say there are two pigments

21 October, 2012
Deepmala Sharma:
hi,sir,I am a final year post-graduate student from University of Mumbai,maharashtra,India.I am wrking on Pseudomonas aeruginosa isolated from Clinical isolates.I wanted to wrk on the pigments produced by the organism.can u kindly help me with some or papers or source i could refer for some useful uses of the pigments .Kindly help.Regards.






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