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Research Journal of Microbiology
  Year: 2007 | Volume: 2 | Issue: 12 | Page No.: 933-939
 
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Effect of Minerals and Moisture Level on the Solid Cultures of A. niger and T. viride for Extracellular Cellulolytic and Xylanolytic Enzymes Production

Muhammad Mohsin Javed, Tehmina Saleem Khan and Ikram-ul- Haq

Abstract:
Solid-state fermentation is the method of choice for generating the industrially important enzymes. Carbon containing compounds in the agricultural wastes are broken down by the microorganisms, which produce the value added commercial enzymes. Thus, the present study describes the extracellular enzymes production like carboxymethyl cellulase (CMC-ase), avicelase, β-glucosidase and xylanase by Aspergillus niger and Trichoderma viride. Agricultural wastes like wheat bran, sugarcane bagasse, corncob, dried grass and rice bran were used under solid-state fermentation conditions. It was found that both the fungal strains have potential to utilize these wastes efficiently as carbon sources and secrete the extracellular proteins possessing hydrolytic ability for the breakdown of these substrates. Culture extract analysis of both strains showed that excreted protein has CMC-ase, avicelase, β-glucosidase and xylanase activity. However, their rate and profile was somehow different, indicating the preference of these strains towards substrate degradability. Additionally, the effect of mineral salts solution with different ratios (v/w) was also investigated and compared with distilled water. Results indicated that Eggins and Pugh minerals salt solution (2:1 ratio) in the presence of corncob as carbon source was best for the bio-synthesis of extracellular hydrolytic enzymes. Moreover, CMC-ase activity 2.24 U mL-1 min-1 in the culture filtrate of T. viride, while avicelase (1.64 U mL-1 min-1), β-glucosidase (4.47 U mL-1 min-1) and xylanase (164.6 U mL-1 min-1) were found to be more active, when A. niger was grown on corncob.
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How to cite this article:

Muhammad Mohsin Javed, Tehmina Saleem Khan and Ikram-ul- Haq, 2007. Effect of Minerals and Moisture Level on the Solid Cultures of A. niger and T. viride for Extracellular Cellulolytic and Xylanolytic Enzymes Production. Research Journal of Microbiology, 2: 933-939.

URL: https://scialert.net/abstract/?doi=jm.2007.933.939

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