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Research Journal of Microbiology
  Year: 2007 | Volume: 2 | Issue: 4 | Page No.: 337-345
DOI: 10.3923/jm.2007.337.345
 
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Comparative Studies of Conjugated Capsular Polysaccharide of Neisseria meningitidis Serogroup A with Outer Membrane Vesicle of Neisseria meningitidis Serogroup B

S.D. Siadat , D. Norouzian , B. Tabaraie , H. Ahmadi , Q. Behzadiyannejad , S.N. Pirayeeh , M. Nejati and B.A. Motlagh

Abstract:
In this study, polysaccharide obtained from Neisseria meningitidis serogroup A was purified according to published protocol of World Health Organization. The outer Membrane Vesicle (OMV) of meningococcal serogroup B was also extracted by ultracentrifuge employing deoxycholate reagent. The derivatives of polysaccharide of meningococcal serogroup A (GAMP) was conjugated to OMV of meningococcal serogroup B which was treated by 1-ethyl-3- (3 dimethylaminopropyl) carbodiimide (EDAC).The obtained polysaccharide was activated by cyanogen bromide (CNBr) and 1-cyano-4-dimethy laminopyridinium tetrafluoroborate (CDAP) separately. First Adipic acid dihydrazide (ADH) with EDAC was bound to GAMP activated with CNBr to form GAMPCNBr AH. Second ADH with EDAC was bound to GAMP activated with CDAP to form GAMPCDAP AH. Then these derivatives were conjugated to OMV by EDAC to form GAMPCNBr AH-OMV and GAMPCDAP AH-OMV. The yields of GAMPCDAPAH-OMV was 45 to 48% which was higher than that of the other prepared conjugate (15-17%). The glycoconjugates were shown to induce hyper immunity in rabbits and formed antibodies against the above mentioned conjugates were detected by immunodiffusion technique.
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How to cite this article:

S.D. Siadat , D. Norouzian , B. Tabaraie , H. Ahmadi , Q. Behzadiyannejad , S.N. Pirayeeh , M. Nejati and B.A. Motlagh , 2007. Comparative Studies of Conjugated Capsular Polysaccharide of Neisseria meningitidis Serogroup A with Outer Membrane Vesicle of Neisseria meningitidis Serogroup B . Research Journal of Microbiology, 2: 337-345.

DOI: 10.3923/jm.2007.337.345

URL: https://scialert.net/abstract/?doi=jm.2007.337.345

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