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Journal of Applied Sciences
  Year: 2020 | Volume: 20 | Issue: 1 | Page No.: 44-50
DOI: 10.3923/jas.2020.44.50
 
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Ethyl Acetate Root Extract of Terminalia glaucescens Protects Drosophila melanogaster Against Virulent Aspergillus Species
Turaki Rifkatu Manasseh, Longchi Satkat Zacchaeus, Dafam Dalen Gwatau, Kakjing Dadul Falang and Ponchang Apollos Wuyep

Abstract:
Background and Objective: Fungal pathogens especially, Aspergillus fumigatus and Aspergillus flavus are the main fungi implicated in many fungal diseases of plants and animals. The present study determined the antifungal activity of ethyl acetate root extract of Terminalia glaucescens in vitro against Aspergillus fumigatus and Aspergillus flavus and in vivo against A. fumigates in Drosophuila melanogaster. Materials and Methods: In the in vitro evaluation, antifungal potentials of the extract were measured using the agar well diffusion method. Survival of Drosophila melanogaster (Drs-LacZ) challenged with A. fumigatus by ingestion method was used to evaluate in vivo activity. Results: Antifungal activity increased in a concentration dependent manner. There was increased activity with increased concentrations. Aspergillus fumigatus was more susceptible to ethyl acetate extract of the roots of T. glaucescens with zones of inhibition when compared with A. flavus at the same concentrations. Zones of inhibition recorded with Itraconazole at different doses against A. flavus and A. fumigatus. MIC of 12.5 mg mL1 was recorded against both organisms with Itraconazole and ranged from 62.25-125 mg mL1 with the ethyl acetate root extract. Survival rate increased by more than 50% in flies treated with ethyl acetate root extract of T. glaucescens. Conclusion: The antifungal results in vitro and in vivo validate the use of this plant in traditional medicine in the treatment of fungal infections. These results have stressed the need for isolation and characterization of the active compound and the elucidation of the mechanisms of action of secondary metabolites on the immune response of Drosophila melanogaster.
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How to cite this article:

Turaki Rifkatu Manasseh, Longchi Satkat Zacchaeus, Dafam Dalen Gwatau, Kakjing Dadul Falang and Ponchang Apollos Wuyep, 2020. Ethyl Acetate Root Extract of Terminalia glaucescens Protects Drosophila melanogaster Against Virulent Aspergillus Species. Journal of Applied Sciences, 20: 44-50.

DOI: 10.3923/jas.2020.44.50

URL: https://scialert.net/abstract/?doi=jas.2020.44.50

 
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