Subscribe Now Subscribe Today
Science Alert
Curve Top
Journal of Applied Sciences
  Year: 2019 | Volume: 19 | Issue: 2 | Page No.: 96-100
DOI: 10.3923/jas.2019.96.100
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

Prevalence of H. pylori Infection among Endoscopy Patients at a Hospital in Makurdi, Nigeria, Using 16s rRNA PCR

Yaji Eunice Mnena , Umeh Uchenna Ebele and Sar Terdzungwe Thaddaeus

Background and Objective: Helicobacter pylori infection is currently recognized worldwide as one of the most common chronic bacterial infections and causes of gastritis, peptic ulcer, gastric cancers and gastric malt lymphoma. It is a debilitating infection, reducing drastically the quality of life. Materials and Methods: The H. pylori were isolated from gastric biopsy samples obtained from the antrum of 80 consenting patients referred for endoscopy at Benue state University Teaching Hospital, (BSUTH), Makurdi, Benue state, Nigeria, placed in Brain Heart Infusion (BHI) broth and tested by both universal bacterial Polymerase Chain Reaction (ubPCR) and 16S rRNA gene (specific to H. pylori ) PCR . Genomic Deoxyribonucleic Acid (gDNA) was extracted from the biopsy samples using ReliaPrep spin column method and used for singleplex PCR. Results: Out of the 80 biopsy specimen collected, 24 (30%) had H. pylori infection as detected by 16S rRNA gene PCR. Using Chi-Square ((χ2) test, there was no significant association between H. pylori infection and gender (χ2= 2.360, p = 0.124). However, significant associations were found between infection and age (χ2= 11.494, p = 0.009) and location of subjects (OR = 2.922, 95% CI: 1.027-8.313, p = 0.040). The findings of this study show high prevalence of Helicobacter pylori in gastritis patients in Makurdi, Benue state. Conclusion: Medical personnel handling cases of gastritis should consider H. pylori infection in the first line of treatment, especially as most illnesses are treated presumptively and symptomatically in most hospitals in this region, without recourse to rigorous laboratory tests. Further, education and public awareness highlighting the relevance of hygiene should be stepped up to help reduce infection rates.
PDF Fulltext XML References Citation Report Citation
How to cite this article:

Yaji Eunice Mnena, Umeh Uchenna Ebele and Sar Terdzungwe Thaddaeus, 2019. Prevalence of H. pylori Infection among Endoscopy Patients at a Hospital in Makurdi, Nigeria, Using 16s rRNA PCR. Journal of Applied Sciences, 19: 96-100.

DOI: 10.3923/jas.2019.96.100






Curve Bottom