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International Journal of Zoological Research
  Year: 2005 | Volume: 1 | Issue: 1 | Page No.: 59-65
DOI: 10.3923/ijzr.2005.59.65
Isolation and Partial Characterization of a Lectin from the Internal Organs of the Sea Cucumber (Holothuria scabra Jaeger)
Elmer-Rico E. Mojica and Florinia E. Merca

Abstract:
The internal organs of the sea cucumber (Holothuria scabra Jaeger) were used as source of lectin. Lectin was isolated by extraction with 0.01 M Tris buffer (0.15 M NaCl at pH 7.5), ammonium sulfate fractionation and gel chromatography using Sephadex G-200. The isolated lectin was found to be non-blood type specific and non-blood group specific since it agglutinated all types of human blood as well as animal erythrocytes. Trypsin and calcium ions increase the isolated lectin’s agglutinating activity. Optimum activity of the lectin was achieved between 20-50°C and at pH 6-8. Hapten inhibition studies did not show any specificity towards the nineteen sugar standards tested. The lectin is a glycoprotein containing 1.33% total sugars as determined by the phenol-sulfuric acid method. The molecular weight of the native lectin was estimated to be 355 kD using Sephadex G-200. SDS-PAGE revealed five sub-units with estimated molecular weights of 120, 98, 80, 65 and 51 kD.
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How to cite this article:

Elmer-Rico E. Mojica and Florinia E. Merca , 2005. Isolation and Partial Characterization of a Lectin from the Internal Organs of the Sea Cucumber (Holothuria scabra Jaeger). International Journal of Zoological Research, 1: 59-65.

DOI: 10.3923/ijzr.2005.59.65

URL: https://scialert.net/abstract/?doi=ijzr.2005.59.65

 
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