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International Journal of Virology
  Year: 2016 | Volume: 12 | Issue: 1 | Page No.: 18-25
DOI: 10.3923/ijv.2016.18.25
Serological and Molecular Identification of Tobacco streak ilarvirus Infecting Onion (Allium cepa L.) on Commercial Fields in Southern India
Sujitha Asadhi, Bommu Veera Bhaskara Reddy, Sivaprasad Yeturu, Usha Rayalcheruvu and T. Giridhara Krishna Timmavajjula

Abstract:
Tobacco streak virus (TSV), a member of the genus Ilarvirus (family: Bromoviridae) is an important viral pathogen infecting onion in Southern India. TSV isolates naturally infecting onion plants were collected from Andhra Pradesh, Tamil Nadu and Karnataka States in South India and characterized. The TSV infection was confirmed by direct antigen coating enzyme linked immunosorbent assay (DAC-ELISA) using TSV antiserum. The CP gene was further amplified by using TSV coat protein primers. The amplicon (717 bp) was cloned, sequenced and sequence analysis revealed that the N gene shared 98.6-100 and 98.3-100% sequence identity with TSV at the nucleotide and amino acid levels, respectively. The phylogenetic relation based on the sequence of these isolates from different geographical regions was also analyzed in this study.
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How to cite this article:

Sujitha Asadhi, Bommu Veera Bhaskara Reddy, Sivaprasad Yeturu, Usha Rayalcheruvu and T. Giridhara Krishna Timmavajjula, 2016. Serological and Molecular Identification of Tobacco streak ilarvirus Infecting Onion (Allium cepa L.) on Commercial Fields in Southern India. International Journal of Virology, 12: 18-25.

DOI: 10.3923/ijv.2016.18.25

URL: https://scialert.net/abstract/?doi=ijv.2016.18.25

 
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