Subscribe Now Subscribe Today
Science Alert
 
FOLLOW US:     Facebook     Twitter
Blue
   
Curve Top
International Journal of Virology
  Year: 2012 | Volume: 8 | Issue: 3 | Page No.: 258-270
DOI: 10.3923/ijv.2012.258.270
Strains of Moroccan watermelon mosaic virus Isolated from Lagenaria breviflorus and Coccinia barteri in Calabar, Southeastern Nigeria
A.T. Owolabi, F. Rabenstein, F. Ehrig, M. Maiss Edgar and H.J. Vetten

Abstract:
Virus infection of Lagenaria breviflora and Coccinia barteri were observed in Calabar, Nigeria during the 2005/2006 growing season. The viruses causing the diseases were characterized in this study. Diagnostic tools were host range, transmission studies, cytopathology, electron microscopy, Immunosorbent Electron Microscopy (ISEM), serology and coat protein gene sequencing. Evidence from biological, serological and sequence data confirmed that both viruses belong to the genus Potyvirus, family Potyviridae. Both were mechanically transmissible and also transmitted by Myzus persicae and Aphis gossyppi in a fore-gut manner. They also induced cytoplasmic inclusions in infected leaf tissues. The Lagenaria virus isolate has a coat protein molecular weight of 32.5 kDa and 35.0 kDa for Coccinia isolate. Of the two viruses only Lagenaria virus isolate showed cross reactivity with MWMV in DAS-ELISA. Both viruses reacted negatively with antisera to some notable cucurbit viruses in the same test, showed weak to moderate decorations with antisera to PRSV, TuMV and TeMV in immunosorbent electron microscopy (ISEM) tests. The Coccinia virus was, however, strongly decorated by antiserum to MWMV but no decoration with the Lagenaria virus. Comparison of the amino acid sequence data of the N-terminal regions of the coat proteins to that of MWMV reported from Sudan indicated 92% and 93% identities for the Coccinia and Lagenaria viruses, respectively. It is suggested that the virus isolates reported in the study be considered strains of the MWMV Sudan isolate. This is the first report of the occurrence of MWMV in Nigeria.
 [Fulltext PDF]   [Fulltext HTML]   [XML: Abstract + References]   [References]   [View Citation]  [Report Citation]
 RELATED ARTICLES:
  •    Physiological and Biochemical Changes in Cucurbita moschata Duch. Ex. Poir Inoculated with a Nigerian Strain of Moroccan Watermelon Mosaic Virus (MWMV): Lagenaria breviflora Isolate
  •    Transmission Efficiency of Two Strains of Moroccan Watermelon Mosaic Virus by Two Clones of Aphis spiraecola (Patch)
  •    Double Infection of a Cucurbit Host by Zucchini Yellow Mosaic Virus and Cucumber Mosaic Virus
  •    First Report of Coat Protein Sequence of Cucumber Green Mottle Mosaic Virus in Cucumber Isolated from Khorasan in Iran
  •    Comparative Biological and Molecular Variability of Zucchini yellow mosaic virus in Iran
  •    Studies on Spread and Identification of Zucchini Yellow Mosaic Virus Disease in the North-West Mediterranean Region of Turkey by Biological Indexing and Double-stranded RNA Analysis
How to cite this article:

A.T. Owolabi, F. Rabenstein, F. Ehrig, M. Maiss Edgar and H.J. Vetten, 2012. Strains of Moroccan watermelon mosaic virus Isolated from Lagenaria breviflorus and Coccinia barteri in Calabar, Southeastern Nigeria. International Journal of Virology, 8: 258-270.

DOI: 10.3923/ijv.2012.258.270

URL: https://scialert.net/abstract/?doi=ijv.2012.258.270

 
COMMENT ON THIS PAPER
 
 
 

 

 
 
 
 
 
 
 
 
 

 
 
 
 
 
 
 
 

Curve Bottom