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International Journal of Virology
  Year: 2009 | Volume: 5 | Issue: 1 | Page No.: 28-35
DOI: 10.3923/ijv.2009.28.35
Isolation and Characterization of PI-3 Virus from Sheep and Goats
Jehan A.M. Gafer, H.A. Hussein and I.M. Reda

Abstract:
The present study was directed to isolate the PI-3 virus from sheep and goats which naturally affected with respiratory manifestation and to identify the isolated virus using several assays. Two hundred and five nasal swab samples were collected from diseased sheep and goats for the isolation of PI-3 virus. The samples were taken from diseased animal at  7  different Governorates Kaffer el sheikh, Alexandria, EL Behaira, Port Said, Demiatta El-Qalubia and Giza through the winter seasons of years 1999 to 2003. The virus was successfully isolated from three ovine samples after three successive passages on MDBK cells. The isolated viruses were then titrated and identified using different biological, serological and molecular assay.
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How to cite this article:

Jehan A.M. Gafer, H.A. Hussein and I.M. Reda, 2009. Isolation and Characterization of PI-3 Virus from Sheep and Goats. International Journal of Virology, 5: 28-35.

DOI: 10.3923/ijv.2009.28.35

URL: https://scialert.net/abstract/?doi=ijv.2009.28.35

 
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