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International Journal of Virology

Year: 2005 | Volume: 1 | Issue: 1 | Page No.: 41-41
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Authors

A. A. EI-Kholy

S. Vilcek

A. M. Daoud

Keywords

Research Article

Phylogenetic Characterization of Some Bovine Viral Diarrhea viruses in Egypt

A. A. EI-Kholy, S. Vilcek and A. M. Daoud
Sixty seven clinically suspected bovine buffy coat specimens, obtained from farms located in Belbees, El-Sharquia, were screened for BVDV. Only 9/67 of the tested specimens (13 %) were BVDV positive by reverse transcription-polymerase chain reaction (RT-PCR). Whereas, virus isolation followed by immunostaining procedure in cell culture confirmed only 6/9 BVI)Vs that were all cytopathogenic. Direct sequencing of the RT-PCR amplicons revealed an extreme nucleotide sequence homology (98%) among all tested Egyptian isolates versus the local vaccinal strain. Sequence alignments showed variable identity (74%-93%) of the Egyptian vaccinal strain versus reference laboratory and vaccinal strains of BVDV. Clustering and phylogenetic analyses suggested that these Egyptian vaccinal and field BVI)Vs are low-Kinetic variants of reference BVDV-1a, particularly the NADL, represented as a distinguished branch within the phylogenetic tree. Accordingly, BVDV-1a (NADL-like), is the dominant circulating genotype in Belbees, El-Sharquia, to date, and the local vaccine could likely induce an antigenically appropriate immune response. The present study offers a useful molecular epizootiological tool to group local BVDVs in a herd-specific manner at a geographic region, to recognize new virus introduction and thus to improve local vaccines and diagnostic assays for effective control policies.
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