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International Journal of Pharmacology

Year: 2018 | Volume: 14 | Issue: 1 | Page No.: 83-92
Research Article

Inhibitory Effects of Sulforaphane on Polyphosphate-mediated Septic Responses

Seongdo Jeong
College of Pharmacy, Cell and Matrix Research Institute, Research Institute of Pharmaceutical Sciences,BK21 Plus KNU Multi-Omics based Creative Drug Research Team, Kyungpook National University
Sae-Kwang Ku
Department of Anatomy and Histology, College of Korean Medicine, Daegu Haany University, 38610 Gyeongsan, Republic of Korea
Jong-Sup Bae
College of Pharmacy, Cell and Matrix Research Institute, Research Institute of Pharmaceutical Sciences,BK21 Plus KNU Multi-Omics based Creative Drug Research Team, Kyungpook National University
LiveDNA: 82.16434
Background and Objective: Sulforaphane (SFN), a natural isothiocyanate present in cruciferous vegetables, such as broccoli and cabbage, is effective in preventing carcinogenesis, diabetes and inflammatory responses. Human endothelial cell-derived polyphosphate (PolyP) is a proinflammatory mediator. This study was undertaken to investigate whether SFN can modulate PolyP-mediated inflammatory responses in Human Umbilical Vein Endothelial Cells (HUVECs) and mice. Materials and Methods: The anti-inflammatory activities of SFN were determined by measuring the permeability, leukocyte adhesion and migration and activation of proinflammatory proteins in PolyP-activated HUVECs and mice. In addition was determined the beneficial effects of SFN on survival rate in PolyP-injected mice. Statistical relevance was determined by one-way analysis of variance (ANOVA) and Tukey’s post-test. Results: Data showed that SFN inhibits PolyP-mediated barrier disruption, cell adhesion molecule expression and leukocyte to HUVEC adhesion/migration. Notably, PolyP-induced NF-κB activation and TNF-α and IL-6 production were inhibited by SFN in HUVECs. These anti-inflammatory functions of SFN were confirmed in PolyP injected mice. Conclusion: Based on the anti-inflammatory effects of SFN in PolyP-mediated septic response, SFN could be considered a potential agent for the treatment of sepsis and other diseases in which PolyP is viewed as a therapeutic target.
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