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International Journal of Pharmacology

Year: 2015 | Volume: 11 | Issue: 5 | Page No.: 477-483
Research Article

Phytochemical Screening, Hypoglycemic and Antihyperglycemic Effect of Flavonoids from the Leaves of Algerian Olea europaea L. in Normal and Alloxan-Induced Diabetic Rats

N. Benhabyles
Laboratory of Biology and Animal Physiology, Normal Superior School, Kouba, 16308, Algeria Faculty of Biological Sciences, University of Science and Technology, Houari Boumediene (USTHB) Bp 32, 16111 Bab Ezzouar EL-Alia, Algier’s, Algeria
K. Arab
Laboratory of Valorisation and Conservation of Biological Resources, Department of Biology, Faculty of Sciences, University of M’Hamed Bougara, Boumerdes, 35000, Algeria
O. Bouchenak
Laboratory of Valorisation and Conservation of Biological Resources, Department of Biology, Faculty of Sciences, University of M’Hamed Bougara, Boumerdes, 35000, Algeria
A. Baz
Laboratory of Biology and Animal Physiology, Normal Superior School, Kouba, 16308, Algeria
Phytotherapy has a promising future in the management of diabetes, considered to be less toxic and free from side effects as compared to the use of synthetic drugs. The aim of this study was set out to study the preliminary phytochemical profile and to determine the hypoglycemic and antihyperglycemic activities of the Aqueous Extract (AE) extracted from the leaves of Olea europaea L. in normal and alloxan-induced diabetic rats. The aqueous extract was obtained by confrontation with organic solvents method with a yield of 37%. The total phenol and flavonoid contents were determined using designed methods and found to be 378.4±5.2 mg/100 g of gallic acid and 125.13±2.8 mg/100 g of quercetin, respectively. The 100 mg kg-1 of aqueous extract was administered intravenously and produced a significant decrease in blood glucose level (p<0.05). Plasma insulin levels were also determined and the results showed a significant increase of insulinemia (p<0.001). In the other hand, Change in blood glucose in diabetic rats treated in acute treatment (2 h) at doses of 200, 400 and 600 mg kg-1 in subchronic treatment (28 days) at a dose of 200 mg kg-1 showed a substantial decrease in blood glucose for all groups treated with different doses of flavonoids in both antidiabetic assays (p<0.001). From the results it can be concluded that flavonoids of Olea europaea can be a potential candidate in treating the hyperglycemic conditions.
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How to cite this article

N. Benhabyles, K. Arab, O. Bouchenak and A. Baz, 2015. Phytochemical Screening, Hypoglycemic and Antihyperglycemic Effect of Flavonoids from the Leaves of Algerian Olea europaea L. in Normal and Alloxan-Induced Diabetic Rats. International Journal of Pharmacology, 11: 477-483.

DOI: 10.3923/ijp.2015.477.483

URL: https://scialert.net/abstract/?doi=ijp.2015.477.483

Comments

HAMZEH ABUNAB Reply

this article is so interested, I'd like to ask your help getting the raw data of the mean and SD of the figure number 5 : change in blood glucose in diabetic rat during 28 days of treatment by AE.

Thank you so much

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