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International Journal of Cancer Research

Year: 2020 | Volume: 16 | Issue: 1 | Page No.: 35-39
Short Communication

Synergistic Combination of Sulforaphane and Methylseleninic Acid in Inhibiting the Proliferation of MCF-7 Breast Cancer Cells

Hanny Nugrahani
Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Padjadjaran, Jl. Raya Bandung Sumedang KM. 21, Jatinangor 45363,Indonesia
Arif S.W. Kusuma
Department of Biological Pharmacy, Faculty of Pharmacy, Universitas Padjadjaran, Indonesia
Jutti Levita
Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Padjadjaran, Jl. Raya Bandung Sumedang KM. 21, Jatinangor 45363,Indonesia
Rizky Abdulah
Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Padjadjaran, Jl. Raya Bandung Sumedang KM. 21, Jatinangor 45363,Indonesia
LiveDNA: 62.21288
Background and Objective: Selenium has been reported to improve the anticancer activity of broccoli sprouts and florets. However, the anticancer properties of the combination of selenium and sulforaphane, the active anticancer compound in Broccoli have not been well investigated. This study was conducted to investigate the role of the combination of selenium and sulforaphane in inhibiting the proliferation of MCF-7 cells. Materials and Methods: Methylseleninic acid was used in this study as it can directly convert to monomethylated selenium for exhibiting its anticancer activity. The anti-proliferative properties of both sulforaphane and methylseleninic acid were analyzed separately and in combination with MCF-7 cells using WST assays. The combinations of sulforaphane and methylseleninic acid in ratios of 1:2 and 1:3 were analyzed using the Chou-Talalay method. Results: The 24 h IC50 values of sulforaphane and methylseleninic acid alone against MCF-7 cells were 24.55 and 182.35 μM, respectively. Furthermore, the results of the Chou-Talalay equation suggested that the combinations of sulforaphane and methylseleninic acid in ratios of 1:2 and 1:3 showed combination index values <1 at all tested concentrations. Conclusion: This study suggested that the combinations of sulforaphane and methylseleninic acid exert synergistic antiproliferative properties against the MCF-7 breast cancer cell line.
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