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International Journal of Cancer Research

Year: 2020 | Volume: 16 | Issue: 1 | Page No.: 1-9
Research Article

In vitro Anti-oxidant and Anti-cancer Activity of Tetradesmus acuminatus Microalgae Extract on MCF-7 Human Breast Cancer Cell Line

Mohammed Abdul Mujeeb
Department of Biotechnology and Microbiology, Karnatak University, Dharwad, India
LiveDNA: 91.29340
Ankalabasappa Vedamurthy
Department of Biotechnology and Microbiology, Karnatak University, Dharwad, India
Arun Kashivishwanath Shettar
Department of Applied Genetics, Karnatak University, Dharwad, India
Sridevi Indrajeet Puranik
Department of Zoology, KLES BK College of Arts, Science and Commerce, Chikkodi, India
LiveDNA: 91.31868
Shridhar Ghagane
Department of Urology, KLES Kidney Foundation, KLES Dr. Prabhakar Kore Hospital and Medical Research Centre, Belagavi, India
LiveDNA: 91.23784
Shivasharana Chandrabanda Thimmappa
Department of Biotechnology and Microbiology, Karnatak University, Dharwad, India
LiveDNA: 91.29355
Background and Objective: Microalgae are the vital constituents in food chains of aquatic ecosystems and have been used for human consumption as food and as medicines. The wide diversity of compounds synthesized from different metabolic pathways of fresh water algae provides promising sources of secondary metabolites in the form of phytochemical constituents. These metabolites are very interesting source of producing an herbal medicine to treat diseases like cancer which is a major public health concern. The study aims to determine the phytochemical constituents, antioxidant and anti-cancer activities of Tetradesmus acuminatus algal extraction MCF-7 human breast cancer cell line. Materials and Methods: The dried and dehydrated algae biomass was subjected to extraction by cold maceration method using 5 solvents of increasing polarity from a non-polar (hexane) to a more polar solvent (aqueous). Phytochemical screening was done using different biochemical tests. Quantitative analysis for phenol was determined by Folin-Ciocalteu reagent method. The antioxidant activity was tested using 2, 2-diphenyl-1-picrylhydrazyl, ferric ion reducing power assay. In vitro anti-cancer activity on MCF-7 human breast cancer cell line was evaluated by (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) MTT assay. Results: The phytochemical analysis revealed broad spectrum of bioactive compounds including flavonoids, glycosides, phenols, tannins, fats and oils. Methanol and aqueous extracts exhibited higher phenolic content as compare to ethanol extract. Antioxidant capacities were shown highest in methanol and ethanol aqueous based on the test performed. The methanol and aqueous extracts were found to be selectively cytotoxic in vitro to on MCF-7 human breast cancer cell line with IC50 values 468.31±24.15 and 598.12±12.18 μg mL–1 for MCF-7, respectively, while it had no cytotoxic effect on normal mice embryo fibroblast cells. Conclusion: The results indicate that Tetradesmus acuminatus was a promising antioxidant and anti-cancer agent for MCF-7 human breast cancer cell line. However, further studies are needed to conclude its therapeutic use.
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Mohammed Abdul Mujeeb, Ankalabasappa Vedamurthy, Arun Kashivishwanath Shettar, Sridevi Indrajeet Puranik, Shridhar Ghagane and Shivasharana Chandrabanda Thimmappa, 2020. In vitro Anti-oxidant and Anti-cancer Activity of Tetradesmus acuminatus Microalgae Extract on MCF-7 Human Breast Cancer Cell Line. International Journal of Cancer Research, 16: 1-9.

DOI: 10.3923/ijcr.2020.1.9

URL: https://scialert.net/abstract/?doi=ijcr.2020.1.9

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References

  1. Al-Hajj, M., M.S. Wicha, A. Benito-Hernandez, S.J. Morrison and M.F. Clarke, 2003. Prospective identification of tumorigenic breast cancer cells. Proc. Nat. Acad. Sci. USA, 100: 3983-3988.
    CrossRefDirect Link
  2. Spiegel, D., J.R. Bloom, H.C. Kraemer and E. Gottheil, 1989. Effect of psychosocial treatment on survival of patients with metastatic breast cancer. Lancet, 2: 888-901.
    CrossRefPubMedDirect Link
  3. Costa, L.S., G.P. Fidelis, C.B. Telles, N. Dantas-Santos and R.B.G. Camara et al., 2011. Antioxidant and antiproliferative activities of heterofucans from the seaweed Sargassum filipendula. Mar. Drugs, 92: 952-966.
    CrossRefDirect Link
  4. Ahmad, M.T., M. Shariff, F.M. Yusoff, Y.M. Goh and S. Banerjee, 2020. Applications of microalga Chlorella vulgaris in aquaculture. Rev. Aquacult., 12: 328-346.
    CrossRefDirect Link
  5. Lefranc, F., A. Koutsaviti, E. Ioannou, A. Kornienko, V. Roussis, R. Kiss and D. Newman, 2019. Algae metabolites: From in vitro growth inhibitory effects to promising anticancer activity. Nat. Prod. Rep., 36: 810-841.
    CrossRefDirect Link
  6. Ercolano, G., P. De Cicco and A. Ianaro, 2019. New drugs from the sea: Pro-apoptotic activity of sponges and algae derived compounds. Mar. Drugs, Vol. 17, No. 1.
    CrossRefDirect Link
  7. Abd El-Hack, M.E., S. Abdelnour, M. Alagawany, M. Abdo and M.A. Sakr et al., 2019. Microalgae in modern cancer therapy: Current knowledge. Biomed. Pharmacother., 111: 42-50.
    CrossRefDirect Link
  8. Aslan, S. and I.K. Kapdan, 2006. Batch kinetics of nitrogen and phosphorus removal from synthetic wastewater by algae. Ecol. Eng., 28: 64-70.
    CrossRefDirect Link
  9. Somasekharan, S.P., A. El-Naggar, P.H. Sorensen, Y. Wang and H. Cheng, 2016. An aqueous extract of marine microalgae exhibits antimetastatic activity through preferential killing of suspended cancer cells and anticolony forming activity. Evid.-Based Complement. Altern. Med., Vol. 2016.
    CrossRefDirect Link
  10. Kumaraswamy, B., L.D. Singh, M.R. Babu and B.D. Rao, 2013. Study of algae from freshwater reservoirs of warangal (A.P), India. Nat. Environ. Pollut. Technol., 12: 577-584.
    Direct Link
  11. Lund, J.W.G. and J.F. Talling, 1957. Botanical limnological methods with special reference to the algae. Bot. Rev., 23: 489-583.
    CrossRefDirect Link
  12. Bjelakovic, G., D. Nikolova and C. Gluud, 2013. Antioxidant supplements to prevent mortality. JAMA., 310: 1178-1179.
    CrossRefDirect Link
  13. Chen, W., C. Zhang, L. Song, M. Sommerfeld and Q. Hu, 2009. A high throughput Nile red method for quantitative measurement of neutral lipids in microalgae. J. Microbiol. Methods, 77: 41-47.
    CrossRefDirect Link
  14. Deepti, K., V. Umadevi, G. Vijayalakshmi and B.V. Polarao, 2012. Antimicrobial activity and phytochemical analysis of Morinda tinctoria Roxb. leaf extracts. Asian Pac. J. Trop. Biomed., 2: S1440-S1442.
    CrossRefDirect Link
  15. Wolfe, K., X. Wu and R.H. Liu, 2003. Antioxidant activity of apple peels. J. Agric. Food Chem., 51: 609-614.
    CrossRefDirect Link
  16. Brand-Williams, W., M.E. Cuvelier and C. Berset, 1995. Use of a free radical method to evaluate antioxidant activity. LWT-Food Sci. Technol., 28: 25-30.
    CrossRefDirect Link
  17. Gulcin, I., 2006. Antioxidant and antiradical activities of L-carnitine. Life Sci., 78: 803-811.
    CrossRefDirect Link
  18. Ghagane, S.C., S.I. Puranik, V.M. Kumbar, R.B. Nerli and S.S. Jalalpure et al., 2017. In vitro antioxidant and anticancer activity of Leea indica leaf extracts on human prostate cancer cell lines. Integrat. Med. Res., 6: 79-87.
    CrossRefDirect Link
  19. Puranik, S.I., M.B. Hiremath, R.B. Nerli and S.C. Ghagane, 2018. Evaluation of in vitro antioxidant and anticancer activity of Tabernaemontana divaricata Leaf extracts against T-24 human bladder cancer cell lines. Int. J. Cancer Res., 14: 100-108.
    CrossRefDirect Link
  20. Ghagane, S.C., S.I. Puranik, R.B. Nerli and M.B. Hiremath, 2017. Evaluation of in vitro antioxidant and anticancer activity of Allophylus cobbe leaf extracts on DU-145 and PC-3 human prostate cancer cell lines. Cytotechnology, 69: 167-177.
    CrossRefDirect Link
  21. Puranik, S.I., S.C. Ghagane, R.B. Nerli, S.S. Jalalpure and M.B. Hiremath, 2017. Evaluation of in vitro antioxidant and anticancer activity of Simarouba glauca leaf extracts on T-24 bladder cancer cell line. Pharmacogn. J., 9: 906-912.
    CrossRefDirect Link
  22. Li, Y., M. Horsman, N. Wu, C.Q. Lan and N. Dubois‐Calero, 2008. Biofuels from microalgae. Biotechnol. Progres., 24: 815-820.
    CrossRefDirect Link
  23. Mukti, N.A., S. Sulaiman, S.M. Saad, J.M.H. Basari, M.A. Rahman, W.Z.W. Ngah and Y.A.M. Yusof, 2009. Chlorella vulgaris exhibited antioxidant and antitumour effects against liver cancer in in vivo and in vitro studies. Sains Malaysiana, 38: 773-784.
    Direct Link
  24. De la Noue, J. and N. de Pauw, 1988. The potential of microalgal biotechnology: A review of production and uses of microalgae. Biotechnol. Adv., 6: 725-770.
    CrossRefDirect Link
  25. Paolini, M., G. Cantelli-Forti, P. Perocco, G.F. Pedulli, S.Z. Abdel-Rahman and M.S. Legator, 1999. Co-carcinogenic effect of β-carotene. Nature, 398: 760-761.
    CrossRefDirect Link
  26. Richmond, A., 2004. Handbook of Microalgal Culture: Biotechnology and Applied Phycology. Blackwell, Oxford, UK.
  27. Sheng, J., F. Yu, Z. Xin, L. Zhao, X. Zhu and Q. Hu, 2007. Preparation, identification and their antitumor activities in vitro of polysaccharides from Chlorella pyrenoidosa. Food Chem., 105: 533-539.
    CrossRefDirect Link
  28. Suárez, E.R., R. Syvitski, J.A. Kralovec, M.D. Noseda and C.J. Barrow et al., 2006. Immunostimulatory polysaccharides from Chlorella pyrenoidosa. A new galactofuranan. measurement of molecular weight and molecular weight dispersion by DOSY NMR. Biomacromolecules, 7: 2368-2376.
    CrossRefDirect Link
  29. Tabarsa, M., I.S. Shin, J.H. Lee, U. Surayot, W. Park and S. You, 2015. An immune-enhancing water-soluble α-glucan from Chlorella vulgaris and structural characteristics. Food Sci. Biotechnol., 24: 1933-1941.
    CrossRefDirect Link
  30. Wang, H., G. Cao and R.L. Prior, 1997. Oxygen radical absorbing capacity of anthocyanins. J. Agric. Food Chem., 45: 304-309.
    CrossRefDirect Link
  31. Gopalakrishnan, S. and E. Vadivel, 2011. GC-MS analysis of some bioactive constituents of Mussaenda frondosa Linn. Int. J. Pharm. Bio Sci., 2: 313-320.
    Direct Link

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