Submit Manuscript

International Journal of Cancer Research

Year: 2018 | Volume: 14 | Issue: 2 | Page No.: 100-108
Research Article

Evaluation of in vitro Antioxidant and Anticancer Activity of Tabernaemontana divaricata Leaf Extracts Against T-24 Human Bladder Cancer Cell Lines

Sridevi I. Puranik
Department of Biotechnology and Microbiology, Karnatak University Dharwad, 580003, Karnataka, India
Murigendra B. Hiremath
Department of Biotechnology and Microbiology, Karnatak University Dharwad, K.L.E'S R. L. Science Institute (Autonomous), 590001,Karnataka, India<
LiveDNA: 91.24730
Rajendra B. Nerli
Department of Urology, JN Medical College, KLE Academy of Higher Education and Research (Deemed to be University), JNMC Campus, Nehru Nagar, Belagavi-590010, Karnataka, India
LiveDNA: 91.25081
Shridhar C. Ghagane
Department of Urology, KLES Kidney Foundation, KLES Dr. Prabhakar Kore Hospital and Medical Research Center, Belagavi-590010, Karnataka, India
LiveDNA: 91.23784
Background and Objectives: Tabernaemontana divaricata belongs to family Apocynaceae is an ornamental, flowering, evergreen shrub that is used in traditional medicine for the treatment of urinary disorders, abdominal tumours and dysentery. The aim of the study was to investigate in vitro antitoxicity and anticancer activities from leaf extracts of Tabernaemontana divaricata. Materials and Methods: The present study involves at screening of preliminary phytochemicals, determination of total phenolic contents, evaluation of methanol, ethanol and aqueous leaf extracts of T. divaricata using in vitro 2, 2-Diphenyl-1-picrylhydrazyl (DPPH) assay, ferric ion reducing antioxidant power (FRAP) assay, Phosphomolybdenum (PM) method and evaluation of in vitro anticancer activity on T-24 human bladder cancer cell line. Results: The preliminary screening of phytochemicals revealed a broad spectrum of secondary metabolites present in all five different solvent extracts. Aqueous extract showed high presence of phenols (62.54±0.18 mg g–1 GAE, r2 = 0.9882) among methanol and ethanol extracts in comparison to Gallic acid (80.28±0.14 mg g–1 GAE, r2 = 0.9954). Ethanol extracts exhibited potential antioxidant activity at a concentration of 100 mg mL–1 (62.41±0.67%). The IC50 value was 188.9±07 µg mL–1 for T-24 bladder cancer cell line whiles the standard drug Doxorubicin showed an IC50 value of 0.23 µM mL–1. Morphological variations was observed in cancerous cells undergoing cell shrinkage and membrane blebbing in human bladder cancer cell line T-24 while the extract showed no cytotoxicity towards normal cells MEF-L929. Conclusion: Based on these results it can be concluded that the tested extracts holds significant antioxidant and anticancer properties. However further investigation with lead compounds of T. divaricata on in vivo trials will enable its therapeutic use.
PDF Fulltext XML References Citation

How to cite this article

Sridevi I. Puranik, Murigendra B. Hiremath, Rajendra B. Nerli and Shridhar C. Ghagane, 2018. Evaluation of in vitro Antioxidant and Anticancer Activity of Tabernaemontana divaricata Leaf Extracts Against T-24 Human Bladder Cancer Cell Lines. International Journal of Cancer Research, 14: 100-108.

DOI: 10.3923/ijcr.2018.100.108

URL: https://scialert.net/abstract/?doi=ijcr.2018.100.108

Leave a Comment

Your email address will not be published. Required fields are marked *

Article Trend

Total views 5469

References

  1. Antoni, S., J. Ferlay, I. Soerjomataram, A. Znaor, A. Jemal and F. Bray, 2017. Bladder cancer incidence and mortality: A global overview and recent trends. Eur. Urol., 71: 96-108.
    CrossRefDirect Link
  2. Breyer, J., S. Shalekenov, A. Aziz, B.W.G. van Rhijn and J. Brundl et al., 2017. Increased proliferation as independent predictor of disease recurrence in initial stage pta urothelial bladder cancer. Bladder Cancer, 3: 173-180.
    CrossRefDirect Link
  3. Sanchez, A., M.F. Wszolek, A. Niemierko, R.H. Clayman and M. Drumm et al., 2018. Incidence, clinicopathological risk factors, management and outcomes of nonmuscle invasive recurrence after complete response to trimodality therapy for muscle invasive bladder cancer. J. Urol., 199: 407-415.
    CrossRefDirect Link
  4. Rojas, T.R., G. Bourdy, E. Ruiz, J.P. Cerapio and P. Pineau et al., 2018. Herbal medicine practices of patients with liver cancer in Peru: A comprehensive study toward integrative cancer management. Integr. Cancer Ther., 17: 52-64.
    CrossRefDirect Link
  5. Raj, P., M. Purushothaman, A. Khusro and S.G. Panicker, 2016. In-vitro anticancer and antioxidant activity of gold nanoparticles conjugate with Tabernaemontana divaricata flower SMs against MCF-7 breast cancer cells. Korean Chem. Eng. Res., 54: 75-80.
    Direct Link
  6. Dantu, A.S., P. Shankarguru, D.R. Devi and B.N.V. Hari, 2012. Evaluation of in vitro anticancer activity of hydroalcoholic extract of Tabernaemontana divaricata. Asian J. Pharm. Clin. Res., 5: 59-61.
    Direct Link
  7. Bhadane, B.S., M.P. Patil, V.L. Maheshwari and R.H. Patil, 2018. Ethnopharmacology, phytochemistry and biotechnological advances of family Apocynaceae: A review. Phytother. Res., 32: 1181-1210.
    CrossRefDirect Link
  8. Jensen, W.B., 2007. The origin of the Soxhlet extractor. J. Chem. Edu., 84: 1913-1914.
    CrossRefDirect Link
  9. Ghagane, S.C., S.I. Puranik, R.B. Nerli and M.B. Hiremath, 2017. Evaluation of in vitro antioxidant and anticancer activity of Allophylus cobbe leaf extracts on DU-145 and PC-3 human prostate cancer cell lines. Cytotechnology, 69: 167-177.
    CrossRefDirect Link
  10. Wolfe, K., X. Wu and R.H. Liu, 2003. Antioxidant activity of apple peels. J. Agric. Food Chem., 51: 609-614.
    CrossRefDirect Link
  11. Brand-Williams, W., M.E. Cuvelier and C. Berset, 1995. Use of a free radical method to evaluate antioxidant activity. LWT-Food Sci. Technol., 28: 25-30.
    CrossRefDirect Link
  12. Oyaizu, M., 1986. Studies on products of browning reaction: Antioxidative activities of products of browning reaction prepared from glucosamine. Jpn. J. Nutr. Diet., 44: 307-315.
    CrossRefDirect Link
  13. Prieto, P., M. Pineda and M. Aguilar, 1999. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: Specific application to the determination of vitamin E. Anal. Biochem., 269: 337-341.
    CrossRefDirect Link
  14. Igarashi, M. and T. Miyazawa, 2001. The growth inhibitory effect of conjugated linoleic acid on a human hepatoma cell line, HepG2, is induced by a change in fatty acid metabolism, but not the facilitation of lipid peroxidation in the cells. Biochim. Biophys. Acta (BBA)-Mol. Cell Biol. Lipids, 1530: 162-171.
    CrossRefPubMedDirect Link
  15. Puranik, S.I., S.C. Ghagane, R.B. Nerli, S.S. Jalalpure and M.B. Hiremath, 2017. Evaluation of in vitro antioxidant and anticancer activity of Simarouba glauca leaf extracts on T-24 bladder cancer cell line. Pharmacogn. J., 9: 906-912.
    CrossRefDirect Link
  16. Ghagane, S.C., S.I. Puranik, V.M. Kumbar, R.B. Nerli and S.S. Jalalpure et al., 2017. In vitro antioxidant and anticancer activity of Leea indica leaf extracts on human prostate cancer cell lines. Integrat. Med. Res., 6: 79-87.
    CrossRefDirect Link
  17. Jain, S., A. Jain, N. Jain, D.K. Jain and N. Balekar, 2010. Phytochemical investigation and evaluation of in vitro free radical scavenging activity of Tabernaemontana divaricata Linn. Natl. Prod. Res., 24: 300-304.
    CrossRefDirect Link
  18. Eldahshan, O.A. and M.M. Abdel-Daim, 2014. Phytochemical study, cytotoxic, analgesic, antipyretic and anti-inflammatory activities of Strychnos nux-vomica. Cytotechnology, 67: 831-844.
    CrossRefDirect Link
  19. Ghate, N.B., B. Hazra, R. Sarkar and N. Mandal, 2014. In vitro anticancer activity of Spondias pinnata bark on human lung and breast carcinoma. Cytotechnology, 66: 209-218.
    CrossRefDirect Link
  20. Kumar, A. and S. Selvakumar, 2015. Antiproliferative efficacy of Tabernaemontana divaricata against HEP2 cell line and Vero cell line. Pharmacogn. Mag., 11: S46-S52.
    CrossRefPubMedDirect Link
  21. Liu, Z., Y. Ye, X. Li, S. Guo and L. Jiang et al., 2018. The effects of intra-arterial chemotherapy on bladder preservation in patients with T1 stage bladder cancer. World J. Urol., 36: 1191-1200.
    CrossRefDirect Link
  22. Jiao, L., L. Bi, Y. Lu, Q. Wang, Y. Gong, J. Shi and L. Xu, 2018. Cancer chemoprevention and therapy using chinese herbal medicine. Biol. Proced. Online, Vol. 20, No. 1.
    CrossRefDirect Link

Keywords