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International Journal of Botany

Year: 2010 | Volume: 6 | Issue: 2 | Page No.: 182-186
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Authors

K. Karim

B. Chokri

S. Amel

H. Wafa

H. Richid

D. Nouredine

Keywords

Research Article

Genetic Diversity of Tunisian Date Palm Germplasm Using ISSR Markers

K. Karim, B. Chokri, S. Amel, H. Wafa, H. Richid and D. Nouredine
Aim of the study is the analysis of the genetic diversity among a set of Tunisian date palm varieties. We used different genetic markers generated from selected ISSR primers. Inter Simple Sequence Repeat (ISSR) markers involve Polymerase Chain Reaction (PCR) amplification of DNA using a single primer composed of a microsatellite sequence. ISSR technology rapidly reveals high polymorphic fingerprints and determines genetic diversity. Seven primers were used to cluster 10 date palm varieties and 82 polymorphic markers were sufficient to identify all of the studied varieties. These discrete molecular markers were used to estimate genetic distances among the 10 accessions and to examine their genetic relationships. Data analysis identified phenetic groups that were in agreement with those obtained according to agronomic traits and random amplified polymorphic DNA (RAPD) markers. Among the 12 Simple Sequence Repeat (SSR) motifs tested, the most abundant were AG. Present result provides evidence of divergence between Tunisian varieties that are organized in clusters. However, we are unable to identify all tested genotypes as mono varietal groups.
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