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International Journal of Agricultural Research

Year: 2011 | Volume: 6 | Issue: 2 | Page No.: 163-171
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Authors

Haitham K.A. EL-Amin

Nada B. Hamza

Atif I. Abuali

Keywords

Research Article

Molecular and Agronomical Assessment of Six Wheat (Triticum aestivum L.) Cultivars under Salt-stress Conditions

Haitham K.A. EL-Amin, Nada B. Hamza and Atif I. Abuali
The study was carried out during two seasons 2007/08 and 2008/09, respectively. It was conducted to determine the effect of salinity stress of four concentration levels on six wheat cultivars under green house conditions and to assess their genetic diversity by using RAPD technique. Split-plot design with four replications was used. The results showed no significant differences between salinity treatments for most of the characters under study except for plant height, number of tillers plant-1 and dry shoot weight in the first season and at plant height, number of leaves plant-1, date to maturity, number of spike and grain weight/plant in second season. There were highly significant differences among varieties under salinity treatments for plant height and the number of tillers/plant in both seasons. No significant differences for interaction between varieties and salinity stress for both seasons. Six RAPD primers (OPA01, OPA03, OPA09, OPA13, OPA14 and OPA20) revealed polymorphism among the six wheat cultivars. RAPD markers were highly efficient and showed high variation among the six cultivars studied. The closer varieties genetically in the cluster behaved similarity in their response to salinity tolerance such as Candor and Debira which were genetically closely related as shown by the dendrogram and the second sisters Pohain and Wady Alnile.
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