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International Journal of Agricultural Research
  Year: 2007 | Volume: 2 | Issue: 2 | Page No.: 152-158
DOI: 10.3923/ijar.2007.152.158
 
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Effect of Different Growth Regulators and Source of Carbohydrates on in and ex vitro Rooting of Iranian Myrtle
A. Shekafandeh

Abstract:
Nodal segments of wild Iranian myrtle was used to study the effects of two carbohydrate sources on shoot proliferation and to determine residual effect of different growth regulators and carbohydrate sources on in and ex vitro rooting. Half strength Murashige and Skoog (MS) medium was used for both shoot proliferation and rooting. The highest shoot proliferation (27.3 shoots/explant) observed in medium containing 2 mg L-1 b-benzylamino purine (BA) with 0.2 mg L-1 Indole-3-acetic acid (IAA) (M7) or 0.2 mg L-1 α-naphthaleneacetic acid (NAA) (M5). There were no significant differences between two sources of carbohydrates on shoot proliferation at all growth regulators levels. Maximum root numbers/shoot (3.8) observed on shoots originating from media containing 2 mg L-1 BA and 0.2 mg L-1 NAA on rooting media containing 0.5 mg L-1 IAA. The rate of rooting increased from zero% in non treated shoots to 91.7% when the basal end of shoots were dipped in a solution of 1.5 mg L-1 IAA and 0.31 mg L-1 Indole-3-butyric acid (IBA) for 24 h before culturing in soil mixture. A procedure for ex vitro rooting was proposed in order to decrease the micropropagation cost.
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How to cite this article:

A. Shekafandeh , 2007. Effect of Different Growth Regulators and Source of Carbohydrates on in and ex vitro Rooting of Iranian Myrtle . International Journal of Agricultural Research, 2: 152-158.

DOI: 10.3923/ijar.2007.152.158

URL: https://scialert.net/abstract/?doi=ijar.2007.152.158

 
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