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Biotechnology

Year: 2009 | Volume: 8 | Issue: 3 | Page No.: 365-369
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Authors

L. Imanparast

D. Hassanpanah

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Research Article

Response of Onobrychis Genotypes to PEG 10000 Induced Osmotic Stress

L. Imanparast and D. Hassanpanah
In order to evaluation of drought stress tolerance of seven onobrychis genotype in vitro condition, was done in Ardabil, Iran in 2009. This experiment was performed by use of factorial design on the basis of completely randomized in three replications. A factor includes five osmotic potential levels (0, -3, -6, -9 and -12 bar) and B factor includes seven onobrychis genotypes (Syntetic, Mako Shoot, Osko Asfanjan, Khosro Shahr Tazekand, Osko Askandaran, Ardabil Garjan and Ardabil Hasanbarogh). For making the different osmotic potentials were used the PEG 10000 and distilled water as control. The variance analysis results showed that there is significant difference between drought levels, genotypes and their interaction as attributes such as coleoptile length, germination uniformity and percent, between drought different levels as attribute germination speed. The Synthetic genotype had the most coleoptile length, germination uniformity and germination percentage in -3 bar and control in compare of the other genotypes. Synthetic genotype showed the most tolerance to drought stress in comparison of the other genotypes and selected the tolerant genotype.
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