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Biotechnology
  Year: 2009 | Volume: 8 | Issue: 1 | Page No.: 138-143
DOI: 10.3923/biotech.2009.138.143
Bioprocessing and Scaling-up Cultivation of Bacillus subtilis as a Potential Antagonist to Certain Plant Pathogenic Fungi, III
S. M. Matar, S. A. El-Kazzaz, E. E. Wagih, A. I. El-Diwany, H. E. Moustafa, M. A. El-Saadani, G. A. Abo-Zaid and E. E. Hafez

Abstract:
The antagonistic and inhibitory activity of Bacillus subtilis isolate G-GANA7 (GenBank accession No. EF583053) obtained from Abo-Homos in Egypt, was tested against six fungal isolates belonging to four different genera, Rhizoctonia solani, Helminthosporium sp., Alternaria sp. and Fusarium oxysporum. The processing of B. subtilis isolate G-GANA7 was cultured in 3 L bench-top New Brunswick Scientific BioFlow III bioreactor for producing the maximum yield of biomass and antifungal compound. Fed-batch processes were automated through a computer aided data bioprocessing system AFS-BioCommand multi-process management program to regulate the cell growth rate by controlling interactively the nutrient feed rate, temperature, pH and agitation speed based on dissolved oxygen. In batch cultivation, the process suffered from low yield of cell mass (3.2 g L-1) and antifungal activity because of high initial glucose concentration followed by acetate formation which the causal agent for inhibition of cell growth. Constant and exponential fed-batch strategies were adopted to circumvent this potential problem. Fed-batch cultivation of B. subtilis was conducted at the specific growth rate of 0.13 and 0.1 h-1 for constant and exponential strategies, respectively. High cell density of 12.8 and 14.6 g L-1 for both operations, with an overall biomass yield of 0.45 g g-1 was achieved. The inhibitory activity of antifungal in supernatant reached its maximum value of 2 and 2.2 cm for constant and exponential fed-batch cultivations.
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How to cite this article:

S. M. Matar, S. A. El-Kazzaz, E. E. Wagih, A. I. El-Diwany, H. E. Moustafa, M. A. El-Saadani, G. A. Abo-Zaid and E. E. Hafez, 2009. Bioprocessing and Scaling-up Cultivation of Bacillus subtilis as a Potential Antagonist to Certain Plant Pathogenic Fungi, III. Biotechnology, 8: 138-143.

DOI: 10.3923/biotech.2009.138.143

URL: https://scialert.net/abstract/?doi=biotech.2009.138.143

 
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