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Biotechnology
  Year: 2006 | Volume: 5 | Issue: 4 | Page No.: 514-516
DOI: 10.3923/biotech.2006.514.516
 
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The Characterization of HaeIII Patterns in the Second Exon of the Buffalo MHC Class II DRB Gene
Sahar Ahmed and Othman E. Othman

Abstract:
In this study, we used restriction endonuclease cleavage sites (HaeIII) as tools to study polymorphisms in buffalo exon 2 of DRB gene. Our results showed that the animals with homozygous restriction patterns are present in high percentage (90%) compared with heterozygous restriction patterns (10%). The homozygous restriction pattern at 190 bp illustrated in 16 of 50 animals, the restriction pattern at 219 bp identified as homozygous site in 22 animals, while two tested animals had restriction pattern at 167 bp. The undigested fragment (284 bp) recorded in 5 animals refer to the absence of HaeIII amino acid substitutions. The heterozygous sites at 190, 167 bp and 219, 190 bp were displayed with very low percentage in tested animals. The HaeIII restriction site at 167 bp has correlated with Pro 56 amino acid which is involved in the formation of Antigen-Recognition Site (ARS) of MHC molecule. According to that, the buffalo MHC class II DRB genetic polymorphisms by HaeIII revealed 6 genetic varients which is associated with animo acid substitutions.
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How to cite this article:

Sahar Ahmed and Othman E. Othman , 2006. The Characterization of HaeIII Patterns in the Second Exon of the Buffalo MHC Class II DRB Gene. Biotechnology, 5: 514-516.

DOI: 10.3923/biotech.2006.514.516

URL: https://scialert.net/abstract/?doi=biotech.2006.514.516

 
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