• [email protected]
  • +971 507 888 742
Submit Manuscript
SciAlert
  • Home
  • Journals
  • Information
    • For Authors
    • For Referees
    • For Librarian
    • For Societies
  • Contact
  1. Biotechnology
  2. Vol 5 (3), 2006
  3. 391-403
  • Online First
  • Current Issue
  • Previous Issues
  • More Information
    Aims and Scope Editorial Board Guide to Authors Article Processing Charges
    Submit a Manuscript

Biotechnology

Year: 2006 | Volume: 5 | Issue: 3 | Page No.: 391-403
DOI: 10.3923/biotech.2006.391.403

Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

Article Trend



Total views 255

Authors


Faisal Ali Bin Anwarali Khan


Awang Ahmad Sallehin Awang Husaini


Keywords


  • sago pith residue hampas
  • celulase
  • a-amylase
  • B. amyloliquefaciens UMAS 1002
  • solid state fermentation
Research Article

Enhancing α-Amylase and Cellulase in vivo Enzyme Expressions on Sago Pith Residue Using Bacilllus amyloliquefaciens UMAS 1002

Faisal Ali Bin Anwarali Khan and Awang Ahmad Sallehin Awang Husaini
The effect of Solid State Fermentation (SSF) parameters on the production of extracellular α-amylase and cellulase (endoglucanases) by Bacillus amyloliquefaciens UMAS 1002 using sago pith residue hampas in shaker flasks was investigated in this study. The appropriate incubation period, temperature, pH, agitation speed, inoculum concentration, hampas concentration and additive substrate effect were optimized for enzyme production. The activity of α-amylase and cellulase was at 14.19 and 13.15 IU mL-1, respectively in optimal culture medium. Maximum yield for both enzymes were achieved by employing 4% w/v hampas in 0.2 M citrate buffer at pH 6 and incubated at 40°C for 6 h with agitation speed of 100 rpm. Inoculum concentrations were found to be optimum at 3% v/v and 4% v/v for α-amylase and cellulase, respectively. Enzyme activity was 2.8 (10.80 IU mL-1) and 3.2 (9.38 IU mL-1) fold higher for α-amylase and cellulase respectively when 1% w/v soluble starch was applied as additive substrates with 0.5% hampas. However in optimal media that consist of 4%w/v of hampas, addition of 1% w/v soluble starch intend to inhibit both enzyme productions. Result revealed that temperature, pH and shaking condition were the most significant factors for the production of α-amylase and cellulase enzyme. Temperature influenced enzyme production by affecting the other parameters including bacterial growth, pH, Dissolved Oxygen (DO) and reducing sugars. Nevertheless, shaking condition could affect DO concentration that in turn affected bacterial growth and enzymes production too.
PDF Fulltext XML References Citation

How to cite this article

Faisal Ali Bin Anwarali Khan and Awang Ahmad Sallehin Awang Husaini, 2006. Enhancing α-Amylase and Cellulase in vivo Enzyme Expressions on Sago Pith Residue Using Bacilllus amyloliquefaciens UMAS 1002. Biotechnology, 5: 391-403.

DOI: 10.3923/biotech.2006.391.403

URL: https://scialert.net/abstract/?doi=biotech.2006.391.403

Related Articles

Application of the 2-Cyanoacetamide Method for SpectrophotometricAssay of Cellulase Enzyme Activity
Screening of Bacterial Isolates from Various Microhabitat Sediments of Kotumsar Cave: A Cogitation on Their Respective Benefits and Expected Threats for Complete Biosphere and Tourists
Screening of Bacterial Isolates from Various Microhabitat Sediments of Kotumsar Cave: A Cogitation on Their Respective Benefits and Expected Threats for Complete Biosphere and Tourists
Optimization of Culture Conditions Affecting Fungal Cellulase Production
Cellulase Production by Pycnoporus sanguineus on Oil Palm Residues through Pretreatment and Optimization Study

Leave a Comment


Your email address will not be published. Required fields are marked *

Useful Links

  • Journals
  • For Authors
  • For Referees
  • For Librarian
  • For Socities

Contact Us

Office Number 1128,
Tamani Arts Building,
Business Bay,
Deira, Dubai, UAE

Phone: +971 507 888 742
Email: [email protected]

About Science Alert

Science Alert is a technology platform and service provider for scholarly publishers, helping them to publish and distribute their content online. We provide a range of services, including hosting, design, and digital marketing, as well as analytics and other tools to help publishers understand their audience and optimize their content. Science Alert works with a wide variety of publishers, including academic societies, universities, and commercial publishers.

Follow Us
© Copyright Science Alert. All Rights Reserved