Biological Control of Potato Isolate of Rhizoctonia solani by Streptomyces olivaceus Strain 115


	Biotechnology
	Year: 2005 \| Volume: 4 \| Issue: 2 \| Page No.: 132-138 DOI: 10.3923/biotech.2005.132.138

Biological Control of Potato Isolate of Rhizoctonia solani by Streptomyces olivaceus Strain 115

S. Shahrokhi , G.H. Shahidi Bonjar and I. Saadoun

Abstract: This is the first report of antifungal activity of Iranian actinomycete isolates against Rhizoctonia solani Kuhn AG-3 (Teleomorph: Thanatephorus cucumeris [(Frank) Donk]). Biological control offers an environmentally friendly alternative to the use of antimicrobials for controlling plant diseases. A collection of about 200 actinomycete strains was screened for the ability to produce metabolites that inhibit R. solani growth in vitro. The Streptomyces olivaceus strain 115 showed strong in vitro antagonistic activity against R. solani in agar disc and Well-diffusion methods by producing extracellular antifungal metabolites. The strain No. 115 was propagated in submerged cultures and active crude was prepared upon which some biological characterization performed. The active metabolite(s) is polar, soluble in H₂O and methanol but insoluble in chloroform, dichloromethane or hexane. Thermal inactivation point of active phase of S. olivaceus strain 115 was 80°C. Antifungal active phase of S. olivaceus strain 115 tolerate range of pH (6-9). Antifungal gene from strain 115 may be a useful candidate for genetic engineering of agriculturally important crop for increased tolerance against R. solani.

PDF Fulltext XML References Citation Report Citation

How to cite this article:

S. Shahrokhi , G.H. Shahidi Bonjar and I. Saadoun , 2005. Biological Control of Potato Isolate of Rhizoctonia solani by Streptomyces olivaceus Strain 115. Biotechnology, 4: 132-138.

DOI: 10.3923/biotech.2005.132.138

URL: https://scialert.net/abstract/?doi=biotech.2005.132.138

COMMENT ON THIS PAPER

	Full Name:
	E-mail:
	Comments:

Navigation


		Online First
		Current Issue
		Previous Issues
		Editorial Board
		Submit a Manuscript
		Guide to Authors
		Subscribe to E-alerts

Indexed In


		AGRICOLA
		AGRIS
		ASCI-Database
		Asian Digital Library
		Cambridge Scientific Abstract
		Ei Compendex

Follow @scialert

Science Alert