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Asian Journal of Scientific Research
  Year: 2021 | Volume: 14 | Issue: 1 | Page No.: 6-12
DOI: 10.3923/ajsr.2021.6.12
 
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Isolation and Characterization of Anoxygenic Photosynthetic Bacteria for Reducing Ammonia and Probiotics Candidate

Sumardi , Rochmah Agustrina, Bambang Irawan, Tugiyono , Desfika Ardia Putri and Achmad Arifiyanto

Abstract:
Background and Objective: The accumulation of organic matter in an aquaculture pond system might increase the accumulation of ammonia compounds which made toxic in aquatic organisms. Increased ammonia concentrations affected enzymatic mechanisms in animals, increased oxygen consumption and interfered with oxygen transport. This research was aimed to reduce ammonia accumulation by increasing the rate of ammonia reduction by Anoxygenic Photosynthetic Bacteria (APB) which was also a potential candidate for probiotics. Materials and Methods: Bacteria were isolated from mangrove forests in the Hanura beach of the Pesawaran district in Lampung province. Strains were grown on SWC media at anoxygenic-photosynthetic condition, morphologically characterized, tested for pH resistance, salinity, antibiotic endurance, ability to compete with Vibrio sp. and ability to reduce ammonia. Results: A total of 7 isolates grew well at pH 7 and 10 and 3 and 6% of NaCl concentrations. L1 strain reduced ammonia by 62%. L2 strain was the most resistant to nalidixic acid, streptomycin, chloramphenicol, ampicillin and trimethoprim. Conclusion: Bacterial strain B has the highest competitive ability against Vibrio sp., which was able to survive up to 2.6 log.Σ.cell. Two probiotic candidates were photosynthetic bacteria. They were able to inhibit of Vibrio sp. growth, reduce the ammonia content by 22-33% and resistant into antibiotics.
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How to cite this article:

Sumardi , Rochmah Agustrina, Bambang Irawan, Tugiyono , Desfika Ardia Putri and Achmad Arifiyanto, 2021. Isolation and Characterization of Anoxygenic Photosynthetic Bacteria for Reducing Ammonia and Probiotics Candidate. Asian Journal of Scientific Research, 14: 6-12.

DOI: 10.3923/ajsr.2021.6.12

URL: https://scialert.net/abstract/?doi=ajsr.2021.6.12

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