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Asian Journal of Plant Sciences
  Year: 2013 | Volume: 12 | Issue: 1 | Page No.: 40-45
DOI: 10.3923/ajps.2013.40.45
 
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Production of Flavonoids in Callus Culture of Anthocephalus indicus A. Rich

S. Indu, L. Vijaya, B. Meeta, V. Jossy and C. Naresh

Abstract:
Manipulation of nutrient medium is the most fundamental approach in enhancing the secondary metabolite production in plant cell culture system. The objective of the experiment was to study the effects of plant growth regulators, L-phenylalanine as precursor and sodium chloride stress on flavonoid production in callus culture of Anthocephalus indicus. It was observed that callus cultures grown on WPM medium supplemented with 3 mg L-1 benzyladenine, 100 mg L-1 phenylalanine and 100 mg L-1 sodium chloride produced 77.2±1.92, 104.4±4.39 and 145.31±4.7 mg of flavonoids, respectively per g dry weight. Combined treatment of all the three produced 203±2.82 mg g-1 dry weight of flavonoids in callus culture which is highest than produced by single treatment. Qualitative analysis of total flavonoid in all the callus samples and leaf material was analysed by HPTLC fingerprinting. As Anthocephalus indicus A. Rich. is a medicinally important plant, the present study offers an alternative method for enhanced production of flavonoid in callus culture.
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How to cite this article:

S. Indu, L. Vijaya, B. Meeta, V. Jossy and C. Naresh, 2013. Production of Flavonoids in Callus Culture of Anthocephalus indicus A. Rich. Asian Journal of Plant Sciences, 12: 40-45.

DOI: 10.3923/ajps.2013.40.45

URL: https://scialert.net/abstract/?doi=ajps.2013.40.45

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