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Asian Journal of Plant Sciences
  Year: 2009 | Volume: 8 | Issue: 7 | Page No.: 505-509
DOI: 10.3923/ajps.2009.505.509
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Nano-Silver Particles Eliminate the in vitro Contaminations of Olive ‘Mission’ Explants

A.A. Rostami and A. Shahsavar

Olive has been cultivated from ancient times. The traditional propagation methods of olive are laborious and with low efficiency. Now, in vitro techniques are an alternative for mass propagation of the species. Establishing a sterile culture is the most challenging step of plant tissue culture process. This study was conducted to evaluate the potential of nano-silver for elimination of contaminations in olive Mission single node explants on Murashige and Skoog half strength (MS/2) medium. Thirty days after culture, the percentages of infected and developed explants were calculated. The results demonstrated that 10% Clorox for 10 min after 70% ethanol for 1 min may be used for surface sterilization of olive explants. Nano-silver used as supplementary disinfectant treatments and microcuttings were submerged into various nano-silver solutions or nano-silver was added to the media. Submergence of microcuttings into various nano-silver solutions was effective to control the internal contaminations, but it also caused severe injuries to the explants. Adding nanosilver (4 mg L-1) to media was fully effective to control explants internal contaminations and no harmful effects were observed on explants and their growth. In conclusion, using very low concentrations of nano-silver as a disinfecting agent in plants tissue culture media is recommended.
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How to cite this article:

A.A. Rostami and A. Shahsavar, 2009. Nano-Silver Particles Eliminate the in vitro Contaminations of Olive ‘Mission’ Explants. Asian Journal of Plant Sciences, 8: 505-509.

DOI: 10.3923/ajps.2009.505.509






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