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Asian Journal of Plant Sciences
  Year: 2005 | Volume: 4 | Issue: 2 | Page No.: 123-127
DOI: 10.3923/ajps.2005.123.127
 
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Comparison of Genetic Diversity Based on Total and Sharp Bands of RAPD Data in Wheat

Hossein Ramshini, Mohammad Reza Naghavi and Hoshang Alizadeh

Abstract:
In this study the molecular variation of 21 bread wheat cultivars/lines was assessed using RAPD markers. With 17 primers 213 bands were scored among which 188 were polymorphic. The mean number of bands was 12.5 for each primer. In this research the analyses were done on two groups of bands: total bands and sharp bands. The similarity matrices and dendrograms were obtained using different algorithms. For sharp bands Simple Matching (SM) coefficient led to higher co-phenetic correlation, but for total bands the correlations were higher for Dice and Jaccard's coefficients. For both groups of bands UPGMA was the best method of clustering. The clusters of total bands and sharp bands have a low correlation with each other. The big decrease from the co-phenetic correlations of total bands to those of sharp bands shows that these two kinds of bands may be different from each other. Choosing the sharp bands did not lead to better results. So we recommend that all bands be scored. The PCO analysis could not classify cultivars in definite clusters.
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How to cite this article:

Hossein Ramshini, Mohammad Reza Naghavi and Hoshang Alizadeh, 2005. Comparison of Genetic Diversity Based on Total and Sharp Bands of RAPD Data in Wheat. Asian Journal of Plant Sciences, 4: 123-127.

DOI: 10.3923/ajps.2005.123.127

URL: https://scialert.net/abstract/?doi=ajps.2005.123.127

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