Research Article

**Expression of the Capsicuum annum (Chili) Defensin Gene in Transgenic Tomatoes Confers Enhanced Resistance to Fungal Pathogens**

Z. Zainal, E. Marouf, I. Ismail and C.K. Fei

Plant defensins are a group of pathogenesis-related defense mechanism proteins. Transgenic tomato plants expressing the chili defensin gene under the control of a Cauliflower Mosaic Virus 35S (CaMV 35S) promoter were generated. The 5 kDa peptide, corresponding to the chili defensin protein was detected in the total protein fraction extracted from the transgenic plants. When compared to partially-purified peptide extracts from untransformed tomato plants, those from transgenic plant possessed the ability to reduce the growth of several fungi in vitro. T₂ transgenic plants were selected and tested for resistance against Fusarium sp. and Phytophthora infestans. The transgenic lines were more resistant to infection by these pathogens than the control plants.

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Z. Zainal, E. Marouf, I. Ismail and C.K. Fei, 2009. Expression of the Capsicuum annum (Chili) Defensin Gene in Transgenic Tomatoes Confers Enhanced Resistance to Fungal Pathogens. American Journal of Plant Physiology, 4: 70-79.

DOI: 10.3923/ajpp.2009.70.79

URL: https://scialert.net/abstract/?doi=ajpp.2009.70.79

American Journal of Plant Physiology

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Authors

Z. Zainal

E. Marouf

I. Ismail

C.K. Fei

Keywords

Research Article

**Expression of the Capsicuum annum (Chili) Defensin Gene in Transgenic Tomatoes Confers Enhanced Resistance to Fungal Pathogens**

How to cite this article

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