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Asian Journal of Biological Sciences

Year: 2012 | Volume: 5 | Issue: 2 | Page No.: 86-95
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Authors

Said Saleh

Alfred King-Yin Lam

Yik-Hong Ho

Keywords

Research Article

A Quantitative Study to Evaluate Expression Level and Correlation of Human Telomerase Reverse Transcriptase and Survivin Genes in Colorectal Cancer Patients

Said Saleh, Alfred King-Yin Lam and Yik-Hong Ho
Colorectal cancer is developed in a multi-step process and associated with genetic alterations. Blocking apoptosis is the key factor to unlimited proliferation and immortalization the cells. Telomerase activation acquires the cell immortalization state whereas survivin expression inhibits the programmed cell death process, apoptosis. These two molecules could play a potential role in cancer development and are attractive targets for clinical trials to develop cancer treatment. In this study, RNA was prepared from colorectal tumor and adjacent non-tumor tissues and then transcribed to complementary DNA. Human Telomerase Reverse Transcriptase (hTERT) and survivin mRNA levels were quantitatively measured by real time Polymerase Chain Reaction (PCR) in tumor and non-tumor tissues. Expression levels of both hTERT and survivin mRNA in adenocarcinomas were significantly higher than in non-tumor mucosa (p<0.0001). The expressions of hTERT and survivin mRNA in adenocarcinomas were correlated (p = 0.012). The high levels of hTERT mRNA were often associated to the high levels of survivin mRNA in colorectal carcinomas (p = 0.039). The expressions of hTERT and survivin mRNA in adenocarcinomas were related to the degree of differentiation (hTERT; p = 0.037, survivin; p = 0.034). No difference was found with other clinicopathological features. These findings indicate the role of hTERT and survivin in colorectal carcinogenesis. HTERT and survivin could be used as a potential diagnostic and prognostic marker in colorectal cancer. Successful inhibition of these molecules could lead to the development of a new drug for cancer therapy.
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How to cite this article

Said Saleh, Alfred King-Yin Lam and Yik-Hong Ho, 2012. A Quantitative Study to Evaluate Expression Level and Correlation of Human Telomerase Reverse Transcriptase and Survivin Genes in Colorectal Cancer Patients. Asian Journal of Biological Sciences, 5: 86-95.

URL: https://scialert.net/abstract/?doi=ajbs.2012.86.95

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