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American Journal of Biochemistry and Molecular Biology
  Year: 2017 | Volume: 7 | Issue: 2 | Page No.: 65-78
DOI: 10.3923/ajbmb.2017.65.78
 
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Phenotypic and Molecular Characterization of some Virulence Factors in Multidrug Resistance Escherichia coli Isolated from Different Clinical Infections in Iraq

Ahmed Abduljabbar Jaloob Aljanaby and Qassim Mmuhsin Hashim Alfaham

Abstract:
Objective: The aim of this study was to detect 10 virulence genes in 50 Escherichia coli strains isolated from patients with different infections. Methodology: Different phenotypic assays and multiplex PCR were used to detect virulence genes. Antimicrobial susceptibility testing was performed to 14 antimicrobials types by disk diffusion method. Results: The most common virulence gene was feoB (98%) and at lower prevalence were genes eaeA and stx1 (4%). Escherichia coli strains were highly resistant to most antimicrobials. Conclusion: Escherichia coli strains isolated from patients with bacteremia were highly virulence and more resistance to antimicrobials than those isolated from other sites.
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How to cite this article:

Ahmed Abduljabbar Jaloob Aljanaby and Qassim Mmuhsin Hashim Alfaham, 2017. Phenotypic and Molecular Characterization of some Virulence Factors in Multidrug Resistance Escherichia coli Isolated from Different Clinical Infections in Iraq. American Journal of Biochemistry and Molecular Biology, 7: 65-78.

DOI: 10.3923/ajbmb.2017.65.78

URL: https://scialert.net/abstract/?doi=ajbmb.2017.65.78

COMMENTS
28 April, 2017
Qassim Mmuhsin Hashim Alfaham:
This research is a product of hard work for more than a year, which earned a Master's degree in excellence, thanks to all who helped me and take my hand to the success of this work and thanks to the staff of the American Journal of Biochemistry and Molecular Biology
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