Standardization of DNA Isolation and PCR Protocol for RAPD Analysis of Suaeda sp.


	Asian Journal of Biotechnology
	Year: 2011 \| Volume: 3 \| Issue: 5 \| Page No.: 486-492 DOI: 10.3923/ajbkr.2011.486.492

Standardization of DNA Isolation and PCR Protocol for RAPD Analysis of Suaeda sp.

S. Gurudeeban, T. Ramanathan, K. Satyavani and T. Dhinesh

Abstract: The present study has evaluated the genetic variability among three salt marsh plant belongs to the family of Chenopodiaceae using random amplified polymorphic DNA markers. The method involves a modified Cetyl trimethylammonium bromide extraction, using polyvinyl pyrrolidone while grinding, successive long-term Chloroform: lsoamyalcohol extractions, an overnight RNase treatment with all steps carried out at room temperature. The yield of DNA ranged from 1.5-2.5 μg μL^-1 per gram of the leaf tissue. The technique is ideal for isolation of DNA from different salt marsh plant species and used for Randomly Amplified Polymorphic DNA (RAPD) analysis. RAPD protocol was optimized based on the use of lower concentrations of primer (2 μM) and Taq polymerase (2 units), 50 ng of template DNA, higher concentration of MgCl₂ (2 mM) and an annealing temperature of 37°C, resulted optimal amplification. The results suggest that the optimized protocol for DNA isolation and PCR was appropriate to diversity analysis of salt marsh plants.

PDF Fulltext XML References Citation Report Citation

RELATED ARTICLES:

•	Phylogeny of Indian Rhizophoraceae Based on the Molecular Data from Chloroplast tRNA^LEU_UAA Intergenic Sequences

•	Protein Electrophoresis and DNA in Herbs Produced from Irradiated Ambrosia maritima Seeds Grown under Soil Salinity and Their Resistance to Insect

•	Characterization of Genetic Similarity of a Hampton Road (Virginia) Live Oak (Quercus virginiana Mil. L.) to a Historic Q. virginiana Using Rapd Markers

•	Genetic Identification of Ceriops decandra (Chiru Kandal) using tRNA (Leu) Molecular Marker

•	Genetic Diversity of North American Soybean (Glycine max L.) Cultivars as Revealed by RAPD Markers

•	A Rapid Efficient Method for DNA Isolation from Plants with High Levels of Secondary Metabolites

•	Assessment of Genetic Relationships Between Two Species of Jute Plants Using Phenotypic and RAPD Markers

•	Standardization of DNA Extraction and Optimization of RAPD-PCR Conditions in Garcinia indica

How to cite this article:

S. Gurudeeban, T. Ramanathan, K. Satyavani and T. Dhinesh, 2011. Standardization of DNA Isolation and PCR Protocol for RAPD Analysis of Suaeda sp.. Asian Journal of Biotechnology, 3: 486-492.

DOI: 10.3923/ajbkr.2011.486.492

URL: https://scialert.net/abstract/?doi=ajbkr.2011.486.492

COMMENT ON THIS PAPER

Navigation


		Online First
		Current Issue
		Previous Issues
		Editorial Board
		Submit a Manuscript
		Guide to Authors
		Subscribe to E-alerts

Indexed In


		ASCI-Database
		Asian Digital Library
		Cambridge Scientific Abstract
		Chemical Abstract Services
		Google Scholar

Follow @scialert

Science Alert