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Asian Journal of Biotechnology
  Year: 2010 | Volume: 2 | Issue: 2 | Page No.: 133-138
DOI: 10.3923/ajbkr.2010.133.138
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Scientific Validation of Piper nigrum by HPLC and Anti-oxidative Assay Markers

E. Menghani, S.C. Jain and R. Jain

The aim of the present investigations is to investigate the HPLC profile and antioxidative assay of P. nigrum L. (A-authentic; M-market samples) and its adulterant (Carica papaya L.). This study will be able to establish libraries as HPLC chromatograms, where such sample tracing can be compared with reference as fingerprints in the evaluation of authentication and standardization methods. The Genus Piper is in great demand as it is known for medicinal properties and newer compound formulations are continually appearing in the market, thus, strict quality inspection of these formulation is necessary. Resolution value of P. nigrum genuine (0.09), market sample 1 (0.06), market sample 2 (0.11) and C. papaya (0.06) was calculated. These results with high resolution value of market samples are closure to C. papaya value, thus, indicative of adulteration. Similarly theoretical plates value further make a clear cut demarcation between P. nigrum genuine (0.80), market 1 (0.16), market 2 (0.08) and its adulterant i.e., C. papaya (0.64). The market sample 2 with same theoretical plate value indicates more purity as compared to market sample 1. The qualitative anti-oxidative assay using DPPH spray on TLC plates bearing the spot of various test extracts of P. nigrum. (A and M) and its adulterant (C. papaya) indicates the presence of antioxidant compounds in P. nigrum (A)<P. nigrum (M)<C. papaya. Different samples of P. nigrum A ( RD50 0.15 mg mL-1) and M ( RD50 <1 μg mL-1) and C. papaya (RD50<1 μg mL-1) demonstrated antioxidant properties and compared with the standards (quercetin RD50 0.003 mg mL-1, ascorbic acid RD50 0.006 mg mL-1). During present study, HPLC analysis and anti-oxidative assay have proved to be effective markers in the quality inspection of the authenticated and its adulterants. The present studies revealed that Piper nigrum as pepper powder can be checked for its adulteration with papaya seeds by these two methods which are helpful in generation of quick and easy methods for quality control to check the purity at quarantine levels.
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How to cite this article:

E. Menghani, S.C. Jain and R. Jain, 2010. Scientific Validation of Piper nigrum by HPLC and Anti-oxidative Assay Markers. Asian Journal of Biotechnology, 2: 133-138.

DOI: 10.3923/ajbkr.2010.133.138


29 June, 2011
Dr Varun Gupta:
Hello sir,
Congrats for very genuine article.....vaery informative and useful for the researchers in the field of pharmaceutical science.....
08 October, 2012
Pradnya Wagh:
Congrats sir,this is exhaustive and original work, can you help for other research students for analysis work?
11 October, 2012
Dr. Ekta Menghani:

Thanks for your kind words. Its a matter of great pleasure to help you. If you have any question do ask me.

12 October, 2012
Pradnya Wagh:
Respected mam,
I want to do HPLC of sapota fruit for phenol,tannin& antioxidant to prepare fruit sample for HPLC?




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