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Asian Journal of Biochemistry

Year: 2006 | Volume: 1 | Issue: 1 | Page No.: 75-83
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Authors

Juan Gualberto Colli

Diana Guillen

Luis Carlos Rodriguez

Nadine Mobious

Enrique Castano

Keywords

Transcriptional regulation depends on the appropriate set of positive and negative regulating signals in order to provide the correct gene expression. Studies in eukaryotic gene expression over the last few years have shown that SRBs/Mediator complex is involved in the proper activation of a variety of genes, while NC2 has been shown to be a general repressor of transcription. NC2 is a conserved transcripcional regulator that was originally identified as inhibitor of basal transcription. Here Our initial results show that the repressor NC2 can interact directly with the Mediator in HeLa cells, this interaction provides a new set of interactions between positive and negative cofactors. Furthermore NC2 depletion from HeLa nuclear extract decrease activated transcription from the HIV–LTR1 promoter in vitro but addition of a complex NC2 fraction restores activated transcription. Further dissection showed that NC2 interacts directly with MED21 a subunit of the Mediator complex. The results shown may provide clues to the controversial role that NC2 has on active promoters.
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