Submit Manuscript

Asian Journal of Animal and Veterinary Advances

Year: 2016 | Volume: 11 | Issue: 5 | Page No.: 263-271
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

Article Trend

Total views 64

Authors

A.F. EL-Kholy

M.A. Zayed

M.F. Shehata

M.A.I. Salem

K.A. El-Bahrawy

N. El-Halawany

M.S. Hassanane

M.S. Hassanane

Keywords

Objective: A part of exon 1 for the myogenic factor gene (MYF5) and two regions from the growth hormone gene (GH 3 UTR and GH 5 UTR) were studied in 11 male dromedary camels for Single Nucleotide Polymorphism (SNP) identification. Methodology and Results: The PCR amplification results showed that MYF5 gene produced fragments of 400 bp, while GH 3 UTR and GH 5 UTR produced fragments of 244 and 630 bp, respectively. The MYF5 gene showed a SNP at the nucleotide 377, in which A>T with a frequency of 0.67, the obtained sequences were converted into amino acid sequences. The conversion proved the change in the codon number 377 from T>A and the translation into the amino acids from methionine to lysine. This gene was found to be correlated with both carcass width at brisket and fat thickness of longissimus dorsi. Although the 3 UTR region of GH gene was found to be correlated with neck length, fore shank height and carcass neck length, this genomic part did not show SNP. Finally the 5 UTR region of the GH gene showed two SNPs at the positions 111 (G>A or G>C) with frequencies of 0.73 and 0.27, respectively and one SNP at the nucleotide 380 (G>A) with a frequency of 0.55. This region was found to be associated with many carcass traits and number of fibers. Conclusion: The results prove the possibility of using the studied genes in marker assisted selection programs for meat production in camels.
PDF Fulltext XML References Citation

How to cite this article

A.F. EL-Kholy, M.A. Zayed, M.F. Shehata, M.A.I. Salem, K.A. El-Bahrawy, N. El-Halawany and M.S. Hassanane, 2016. Association of Single Nucleotide Polymorphisms for Myogenic Factor 5 and Growth Hormone Genes with Meat Yield and Quality Traits in One Humped Camel (Camelus dromedarius). Asian Journal of Animal and Veterinary Advances, 11: 263-271.

DOI: 10.3923/ajava.2016.263.271

URL: https://scialert.net/abstract/?doi=ajava.2016.263.271

Leave a Comment

Your email address will not be published. Required fields are marked *