• [email protected]
  • +971 507 888 742
Submit Manuscript
SciAlert
  • Home
  • Journals
  • Information
    • For Authors
    • For Referees
    • For Librarian
    • For Societies
  • Contact
  1. Asian Journal of Animal and Veterinary Advances
  2. Vol 7 (10), 2012
  3. 989-997
  • Online First
  • Current Issue
  • Previous Issues
  • More Information
    Aims and Scope Editorial Board Guide to Authors Article Processing Charges
    Submit a Manuscript

Asian Journal of Animal and Veterinary Advances

Year: 2012 | Volume: 7 | Issue: 10 | Page No.: 989-997
DOI: 10.3923/ajava.2012.989.997

Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

Article Trend



Total views 158

Authors


Jianzhou Shi

Country: China

Guirong Sun

Country: China

Yadong Tian

Country: China

Ruili Han

Country: China

Guoxi Li

Country: China

Yanqun Huang

Country: China

Jianhua Wang

Country: China

Xiangtao Kang

Country: China

Keywords


  • Breast blister (keel cyst)
  • DDRT-PCR
  • ESTs
  • F2 resource population
  • SqRT-PCR
  • SSCP
Research Article

Screening Genes Related to Breast Blister (Keel Cyst) in Chicken by Delta Differential Display

Jianzhou Shi, Guirong Sun, Yadong Tian, Ruili Han, Guoxi Li, Yanqun Huang, Jianhua Wang and Xiangtao Kang
In this study, screening and identification differentially displayed genes related to breast blisters (keel cysts) in the “Gushi chickensxAnkao” F2 resource population. Delta Different Display Reverse Transcription-Polymerase Chain Reaction (DDRT-PCR) in combination with Single Strand Conformation Polymorphism (SSCP) is a very sensitive tool for analyzing gene expression of isolated liver tissue from birds. The special primer pair was designed to identify the differential displayed band and a semi-quantitative Reverse Transcription-Polymerase Chain Reaction (RT-PCR) was performed simultaneously. We now are able to directly characterize genetic differences in breast blisters and the healthy birds. The new ESTs was submitted to GenBank (Accession number: GW317166, GW317167, GW317168, GW317169, GW317170, GW317171, GW836603). Several of the differentially expressed fragments were messenger RNA. Six fragments showed interesting homologies to known sequences from the databases. One fragment did not show any homologies to known sequences. This lays a foundation for further study on the mechanism of differential gene expression in breast blisters and the healthy chickens. It is the first time to demonstrate in this study that Tumor Susceptibility Gene 101 (TSG101) is related to breast blisters (keel cysts) of poultry. Furthermore, GW317167 is suggested to contribute to the development of breast blisters (keel cysts), thus may be candidates of new targets of tumor susceptibility genes.
PDF Fulltext XML References Citation

How to cite this article

Jianzhou Shi, Guirong Sun, Yadong Tian, Ruili Han, Guoxi Li, Yanqun Huang, Jianhua Wang and Xiangtao Kang, 2012. Screening Genes Related to Breast Blister (Keel Cyst) in Chicken by Delta Differential Display. Asian Journal of Animal and Veterinary Advances, 7: 989-997.

DOI: 10.3923/ajava.2012.989.997

URL: https://scialert.net/abstract/?doi=ajava.2012.989.997

Related Articles

Diagnosis of Cereal Viruses in the Middle East
Effect of Pre-incubation Storage of Hatching Eggs on Subsequent Post-hatch Growth Performance and Carcass Quality of Broilers
Nuclear Proteins Associated with Hyphen Growth in Candida albicans
Characterization of SNPs of Bovine Prolcatin Gene of Holstein Cattle
The Effect of Transfer Battery Brooded Broilers on Conventional Rice Husk Littered Floor on Production Performance

Leave a Comment


Your email address will not be published. Required fields are marked *

Useful Links

  • Journals
  • For Authors
  • For Referees
  • For Librarian
  • For Socities

Contact Us

Office Number 1128,
Tamani Arts Building,
Business Bay,
Deira, Dubai, UAE

Phone: +971 507 888 742
Email: [email protected]

About Science Alert

Science Alert is a technology platform and service provider for scholarly publishers, helping them to publish and distribute their content online. We provide a range of services, including hosting, design, and digital marketing, as well as analytics and other tools to help publishers understand their audience and optimize their content. Science Alert works with a wide variety of publishers, including academic societies, universities, and commercial publishers.

Follow Us
© Copyright Science Alert. All Rights Reserved