Subscribe Now Subscribe Today
Science Alert
 
Blue
   
Curve Top
Asian Journal of Animal and Veterinary Advances
  Year: 2012 | Volume: 7 | Issue: 8 | Page No.: 702-709
DOI: 10.3923/ajava.2012.702.709
 
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

Detecting Splicing Variants of FOXP2 and its Protein Expression in Chicken Brain

Xi Wang, Hongxia Li, Mohammod Abdul Hamid and Xingbo Zhao

Abstract:
The whole cDNA sequence of chicken FOXP2 gene was obtained by 3' and 5' RACE and the length was 3839 bp, getting four splicing variants for protein isoforms (707 AA, 706AA, 687AA, 684AA). The four splicing variants were common to Zang chicken, White Plymouth Rock, Beijing fat chicken, White leghorns and Rhode Island Red by the detection of a combination of PCR and sequencing and according to two main splicing variants, two antibodies were produced to be used to detect equivalent expression in chicken brain such as cortex, striatum, mesencephalon, thalamus and hypothalamus, in White leghorns (male and female) by immunohistochemistry. The results showed that different FOXP2 mRNA and protein expression had no spatial difference.
PDF Fulltext XML References Citation Report Citation
 RELATED ARTICLES:
  •    Production and Purification of Polyclonal Antibody Against Human Kappa Light Chain
  •    Immunohistochemical Study of Inducible Nitric Oxide and its Prognosis in Schistosomasis Associated Urinary Bladder Carcinoma
  •    Differential Expression of IL-6 and IGF-II in Guinea Fowl and Chicken
  •    Histochemical and Physicochemical Study of Bacterial Exopolysaccharides
How to cite this article:

Xi Wang, Hongxia Li, Mohammod Abdul Hamid and Xingbo Zhao, 2012. Detecting Splicing Variants of FOXP2 and its Protein Expression in Chicken Brain. Asian Journal of Animal and Veterinary Advances, 7: 702-709.

DOI: 10.3923/ajava.2012.702.709

URL: https://scialert.net/abstract/?doi=ajava.2012.702.709

COMMENT ON THIS PAPER
 
 
 

 

 
 
 
 
 
 
 
 
 

 
 
 
 
 
 
 

Curve Bottom