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Asian Journal of Animal and Veterinary Advances
  Year: 2012 | Volume: 7 | Issue: 4 | Page No.: 299-308
DOI: 10.3923/ajava.2012.299.308
 
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Semen Storage at 23, 4 or -196°C and its Application to Artificial Insemination in Small-tail Han Sheep
Abulizi Wusiman, Yan-Ping Wang, Kang Ren, Guang-Bin Zhou, Xiang-Wei Fu, Lun Suo, Zhi-Qiang Fan, Liang Wang and Shi-En Zhu

Abstract:
In order to provide more options to sheep artificial insemination (AI) technology this study evaluated the influence of preservation methods on sperm quality and subsequent fertility in Small-tail Hun sheep. For the following treatments: Diluted fresh semen as a control, diluted semen stored at 4°C for 24, 48, 72, 96 or 120 h, Separate aliquots of diluted semen were stored at 23°C for 24 or 48 h and diluted semen stored at -196°C, The semen was evaluated for sperm viability, acrosomal integrity, mitochondrial function and capacitation status using Propidium Iodide (PI), fluoresceinated peanut lectin (FITC-PNA), 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolyl-carbocya nine iodide (JC-1) and chlortetracycline staining, respectively. Proportions of spermatozoa showing high mitochondrial membrane potential and an incapacitated status were lower (p<0.05) in frozen semen than in samples stored at either 4°C for 72 h or at 23°C for 24 h. Sperm viability did not differ (p>0.05) among: Fresh semen, semen stored at 4°C for 48 h and semen stored at 23°C for 24 h. Following cervical insemination, semen stored at 4°C for 72 h or at 23°C for 24 h resulted in similar pregnancy rates (p>0.05) to those from fresh sperm (16/23, 69.6%, 19/25, 76.0% and 22/28, 78.6%), all of which were higher (p<0.05) than those from semen cryopreserved at -196°C (12/24, 50.0%). In conclusion, semen stored at either 4°C for 72 h or at 23°C for 24 h is structurally superior to semen cryopreserved at -196°C and achieved similar pregnancy rates as from AI using fresh semen.
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How to cite this article:

Abulizi Wusiman, Yan-Ping Wang, Kang Ren, Guang-Bin Zhou, Xiang-Wei Fu, Lun Suo, Zhi-Qiang Fan, Liang Wang and Shi-En Zhu, 2012. Semen Storage at 23, 4 or -196°C and its Application to Artificial Insemination in Small-tail Han Sheep. Asian Journal of Animal and Veterinary Advances, 7: 299-308.

DOI: 10.3923/ajava.2012.299.308

URL: https://scialert.net/abstract/?doi=ajava.2012.299.308

 
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