Subscribe Now Subscribe Today
Science Alert
 
FOLLOW US:     Facebook     Twitter
Blue
   
Curve Top
Asian Journal of Animal and Veterinary Advances
  Year: 2011 | Volume: 6 | Issue: 12 | Page No.: 1233-1240
DOI: 10.3923/ajava.2011.1233.1240
Comparative Improvement in Function of Isolated Rat Langerhans Islets by Various Phosphodiesterase 3, 4 and 5 Inhibitors
Mojdeh Mohammadi, Shokoufeh Atashpour, Nazila Pourkhalili, Amir Nili-Ahmadabadi, Maryam Baeeri, Azadeh Mohammadirad, Shokoufeh Hassani, Shekoufeh Nikfar and Mohammad Abdollahi

Abstract:
Increased oxidative stress plays a role in the pathogenesis of cellular death and β-cell failure. The aim of this study was to evaluate the action of different types of phosphodiesterase (PDE) inhibitors including milrinone (PDE-3), rolipram (PDE-4) and sildenafil (PDE-5) on viability, production of Reactive Oxygen Species (ROS) and secretion of insulin from isolated rat pancreatic islets. Pancreatic islets were carefully isolated and incubated in RPMI 1640 for 24 h. After overnight incubation, islets were picked up and divided into ten in each groups. Then, milrinone, rolipram and sildenafil at doses of 0.1, 1, 10 and 100 FM were added to islet groups and incubated for further 24 h. Then static insulin secretion at 2.8 and 16.7 mM concentrations of glucose, was tested. Then the viability of cells, level of ROS and insulin were examined. The results of static experiments showed that secretion of insulin increased significantly in response to glucose at both basic (2.8 mM) and stimulation (16.7 mM) levels by the lower doses of tested PDE inhibitors. The level of ROS at the lower doses of milrinone decreased. The viability of islets at the lower doses of all of PDE inhibitors were increased; however, viability at the higher doses of sildenafil and rolipram reduced significantly. Milrinone was the most effective PDE inhibitors on the function of isolated pancreatic islets. PDE inhibitors show the most significant anti-oxidative effects at lower doses. Concerning improvement of isolated islets function, PDE-3 inhibitor is the best among tested compounds. PDE inhibitors may help management of diabetes and facilitate conditions of islet transplantation.
PDF Fulltext XML References Citation Report Citation
 RELATED ARTICLES:
  •    On the Benefit of Pure Glycyrrhizic Acid on the Function and Metabolic Activity of Isolated Pancreatic Langerhans Islets in vitro
  •    Antioxidants as an Appropriate Approach to Improve the Outcome of Pancreatic Islet Isolation: Evidences from Animal Studies
  •    A Review on the Potential Benefits of Phosphodiesterase Inhibitors in Various Models of Toxicities in Animals
  •    A Comprehensive Review of Biochemical and Molecular Evidences from Animal and Human Studies on the Role of Oxidative Stress in Aging: An Epiphenomenon or the Cause
  •    A Systematic Review of the Efficacy and Safety of Teucrium Species; from Anti-oxidant to Anti-diabetic Effects
  •    Effects of Sildenafil a Phosphodiesterase 5 Inhibitor on Rat Liver Cell Key Enzymes of Gluconeogenesis and Glycogenolysis
  •    Positive Response of Isolated Rat Pancreatic Islets to IMOD; Hopes for Better Transplant Outcome and Graft Function
  •    An Update on Pharmacology of Satureja Species; From Antioxidant, Antimicrobial, Antidiabetes and Anti-hyperlipidemic to Reproductive Stimulation
How to cite this article:

Mojdeh Mohammadi, Shokoufeh Atashpour, Nazila Pourkhalili, Amir Nili-Ahmadabadi, Maryam Baeeri, Azadeh Mohammadirad, Shokoufeh Hassani, Shekoufeh Nikfar and Mohammad Abdollahi, 2011. Comparative Improvement in Function of Isolated Rat Langerhans Islets by Various Phosphodiesterase 3, 4 and 5 Inhibitors. Asian Journal of Animal and Veterinary Advances, 6: 1233-1240.

DOI: 10.3923/ajava.2011.1233.1240

URL: https://scialert.net/abstract/?doi=ajava.2011.1233.1240

 
COMMENT ON THIS PAPER
 
 
 

 

 
 
 
 
 
 
 
 
 

 
 
 
 
 
 
 

       

       

Curve Bottom