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Asian Journal of Animal Sciences
  Year: 2016 | Volume: 10 | Issue: 3 | Page No.: 189-195
DOI: 10.3923/ajas.2016.189.195
 
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Genetic Characterization of Open Reading Frame5 (ORF5) of Porcine Reproductive and Respiratory Syndrome Virus in Indonesia Between 2008 and 2014

Faisal Faisal, Muharam Saipulloh, Rini Widayanti , Aris Haryanto and Charles Rangga Tabbu

Abstract:
To investigate the genetic diversity of the Indonesian Porcine Reproductive and Respiratory Syndrome Virus (PRRSV), the complete Open Reading Frame 5 (ORF5) from 11 clinical samples collected between 2008-2014 was sequenced and phylogenetically analyzed. These sequences were compared with the ORF5 of NA referens viruses (VR2332, MLV and JXA1), lelystad virus and 34 ORF5 sequences North America group from Gen Bank. Sequence analysis revealed that the ORF5 have undergone genetic variation. The ORF5 nucleotide and amino acid identities among 11 Indonesia PRRS viruses ranged from 88.5-100 and 87.5-100%, respectively and all belonged to the North American genotype. The ORF5 sequence of nucleotide and amino acids from the North American prototype virus (VR2332) and derived vaccine virus (MLV) were 97.6-100 and 97.0-100% identical to the various ORF5 Indonesia viruses, respectively. In the phylogenetic analysis, of the Indonesian viruses were clusted into two groups. The ORF5 based sequencing analysis can be used to determine the type of PRRS virus and the presence of genetic diversity of PRRS viruses in Indonesia.
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How to cite this article:

Faisal Faisal, Muharam Saipulloh, Rini Widayanti, Aris Haryanto and Charles Rangga Tabbu, 2016. Genetic Characterization of Open Reading Frame5 (ORF5) of Porcine Reproductive and Respiratory Syndrome Virus in Indonesia Between 2008 and 2014. Asian Journal of Animal Sciences, 10: 189-195.

DOI: 10.3923/ajas.2016.189.195

URL: https://scialert.net/abstract/?doi=ajas.2016.189.195

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