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Asian Journal of Agricultural Research
  Year: 2017 | Volume: 11 | Issue: 4 | Page No.: 108-115
DOI: 10.3923/ajar.2017.108.115
 
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Analysis of Chemical Composition of Mulberry Silkworm Pupal Oil with Fourier Transform Infrared Spectroscopy (FTIR), Gas Chromatography/Mass Spectrometry (GC/MS) and its Antimicrobial Property

Param Dev, Venkatesh Kumar Ramappa , Ram Gopal and Sangeeta

Abstract:
Background and Objective: The antibacterial actions of long-chain unsaturated fatty acids are usually attributed to inhibit many pathogenic microorganisms. The objective of the present study was to analyze the chemical composition of pupal oil of mulberry silkworm with fourier transform infrared spectroscopy (FTIR) and Gas Chromatography/Mass Spectrometry (GC/MS) and to evaluate the isolates for possible in vitro antibacterial activity. Materials and Methods: Pupae of the silkworm Bombyx mori were produced at Department of Applied Animal Sciences, Babasaheb Bhimrao Ambedkar University, Lucknow District of Uttar Pradesh, India, by conducting silkworm rearing as per protocol. The silkworm pupal oil was extracted by using maceration method. Further silkworm pupal oil was analyzed with Model name as Nicolet 6700 Trade Mark Spectrometer and GC/MS. The antimicrobial activity was test by Minimum Inhibitory Concentration (MIC) method using Fuente method. The data was analyzed by One-way analysis of variance (ANOVA) using SPSS program. Results: The FTIR analysis of pupal oil proved the presence of alkenes, alkanes, alkynes, organic halogen compounds, aromatic compounds, ethers, esters, aldehydes, alcohols, carboxylic acid, amides and phenols and amides. The GC/MS analysis of pupal oil revealed the existence of the Stearic acid, Palmitic acid and Linoleic acid. Conclusion: The FTIR and GC/MS analysis of pupal oil proved the presence of different useful compounds.
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How to cite this article:

Param Dev, Venkatesh Kumar Ramappa, Ram Gopal and Sangeeta , 2017. Analysis of Chemical Composition of Mulberry Silkworm Pupal Oil with Fourier Transform Infrared Spectroscopy (FTIR), Gas Chromatography/Mass Spectrometry (GC/MS) and its Antimicrobial Property. Asian Journal of Agricultural Research, 11: 108-115.

DOI: 10.3923/ajar.2017.108.115

URL: https://scialert.net/abstract/?doi=ajar.2017.108.115

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