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Asian Journal of Applied Sciences
  Year: 2010 | Volume: 3 | Issue: 4 | Page No.: 250-261
DOI: 10.3923/ajaps.2010.250.261
 
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Measurement of the Viscosity of Mousse Myoblasts Modified with the αβ Cristalline through the Technique of Micromanipulation by the Means of Micropipette

A. Sako, A. Memeledje and P. Assamoi

Abstract:
In this study, we measured directly through the technique of micromanipulation by micropipette the viscosity of mouse muscle cells modified by transfection with the αβ-crystalline in order to highlight the role played by the changes in the cytoskeleton. Two vectors were used, the αβ-crystalline of wild type and R129G αβ-crystalline mutant. The medium and intrinsic cell viscosities were determined and compared. For this purpose, two populations of muscle cell clones were used: WT5, WT6 for cells transfected with the αβ-crystalline of wild type and Mut9, Mut11 for cells transfected with R129G αβ-crystalline mutant. There is a difference between the medium and intrinsic viscosities of these different types of muscle cells of mice.
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How to cite this article:

A. Sako, A. Memeledje and P. Assamoi , 2010. Measurement of the Viscosity of Mousse Myoblasts Modified with the αβ Cristalline through the Technique of Micromanipulation by the Means of Micropipette. Asian Journal of Applied Sciences, 3: 250-261.

DOI: 10.3923/ajaps.2010.250.261

URL: https://scialert.net/abstract/?doi=ajaps.2010.250.261

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