Pei Ma
Shaanxi Key Laboratory of Degradable Biomedical Materials, Northwest University, China
Lingyun Liu
Shaanxi Key Laboratory of Degradable Biomedical Materials, Northwest University, China
Chenhui Zhu
Shaanxi Key Laboratory of Degradable Biomedical Materials, Northwest University, China
Jianjun Deng
Shaanxi Key Laboratory of Degradable Biomedical Materials, Northwest University, China
Yu Mi
Shaanxi Key Laboratory of Degradable Biomedical Materials, Northwest University, China
Xiaoxuan Ma
Shaanxi Key Laboratory of Degradable Biomedical Materials, Northwest University, China
Daidi Fan
Shaanxi Key Laboratory of Degradable Biomedical Materials, Northwest University, China
ABSTRACT
In order to improve Human-like Collagen (HLC) iron binding capacity, the thiolated Human-Like collagen (SH-HLC) was adopted as chelating substrate. The binding of iron to SH-HLC was examined at varying pH, molar ratio (SH-HLC/Fe) and reaction time. The maximum binding capacity of SH-HLC was 31.18 mol iron per mol SH-HLC at pH 7.5, molar ratio 1:500 and reaction time 40 min. In addition, the SH-HLC and human-like collagen iron (SH-HLC-Fe) complex showed no toxicity to BHK cell through cell viability assay. These results suggest that the SH-HLC-Fe complex is a potential compound for use as a iron source.
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How to cite this article
Pei Ma, Lingyun Liu, Chenhui Zhu, Jianjun Deng, Yu Mi, Xiaoxuan Ma and Daidi Fan, 2013. Preparation of a Novel Thiolated Human-like Collagen Iron. Biotechnology, 12: 117-120.
DOI: 10.3923/biotech.2013.117.120
URL: https://scialert.net/abstract/?doi=biotech.2013.117.120
DOI: 10.3923/biotech.2013.117.120
URL: https://scialert.net/abstract/?doi=biotech.2013.117.120
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