Sarder Nasir Uddin
Not Available
Soubir Titov
Not Available
Md. Abdul Wadud
Not Available
ABSTRACT
Shoot tips of a traditional table banana [Musa sp. cv. Kanthali (Genome AAB)] of Bangladesh were evaluated for in vitro propagation. Initial surface sterilization (with 0.1% HgCl2 for 12 min) of shoot tips was successful but microbial contamination (mostly bacteria) at the rhizomatous base of the explants were observed within 6-15 days after inoculation which eventually killed 85% inoculated explants. So, for contamination free culture establishment explants were soaked in two broad spectrum antibiotics namely ampicillin and gentamicin. Cent percent contamination free cultures were established by soaking the explants in 400 mg L-1 ampicillin or 200 mg L-1 gentamicin for 1 h. Antibiotic treated explants were found to be full contamination free but failed to regenerate after 3 weeks of culture. But some of them absorbed media for up to 2nd subculture and showed swelling of explants and some color changes from pale white to light/deep green. Finally, a few days after 3rd subculture, no growth of explants was observed and all treated explants eventually started to die. Among the untreated alive explants the best medium for single shoot development was MS + 4.0 mg L-1 BA + 0.5 mg L-1 Kn + 15% CW and average time required for shoot development was 18-21 days. But the regeneration percentage was very low (30%). The best medium for shoot multiplication was MS + 4.0 mg L-1 BA + 2.0 mg L-1 IAA + 15% CW and average time required for production of multiple shoots from single shoot was 40-45 days. Multiplication rate was also too low (40%) and only average 3-4 shoots were formed. Finally, in vitro proliferated shoots produced roots with maximum frequency (90%) in half strength of MS medium fortified with 0.5 mg L-1 IBA.
PDF References
How to cite this article
Sarder Nasir Uddin, Soubir Titov and Md. Abdul Wadud, 2006. In vitro Propagation of Banana (Musa sp. Cv. Kanthali): An Endogenous Variety of Bangladesh with Controlling Bacterial Contaminations. American Journal of Plant Physiology, 1: 169-176.
DOI: 10.3923/ajpp.2006.169.176
URL: https://scialert.net/abstract/?doi=ajpp.2006.169.176
DOI: 10.3923/ajpp.2006.169.176
URL: https://scialert.net/abstract/?doi=ajpp.2006.169.176
REFERENCES
- Arinaitwe, G., P.R. Rubaihayo and M.J.S. Magambo, 2000. Proliferation rate effects of cytokinins on banana (Musa sp.) cultivars. Sci. Hortic., 86: 13-21.
Direct Link - Bradbury, J.F., 1988. Identification of cultivable bacteria from plants and plant tissue cultures by use of simple classical methods. Acta Hortic., 225: 27-37.
Direct Link - Cronauer, S.S. and A.D. Krikorian, 1984. Rapid multiplication of bananas and plantains by in vitro. Hortic. Sci., 19: 234-235.
Direct Link - Hadiuzzaman, S., U. Habiba, S. Reza, M.L. Saha and M.R. Khan, 2001. Development of a sustainable protocol for contamination free culture of table bananas and identification of associated endogenous bacteria. Proceedings of the 4th International Plant Tissue Culture Confernce, November 1-3, 2001, Dhaka, pp: 24.
- Jarret, R.L., W. Rodriquez and R. Fernandez, 1985. Evaluation, tissue culture propagation and dissemination of Saba and Pelipita plantains in costa rica. Sci. Hortic., 25: 137-147.
Direct Link - Martinez, B.L., 2004. Alternativas Para el Manejo de Ralstonia Solanacearum Raza II, Causante de la Marchitez Bacterial del Banano. In: Memorias del XVI Reunion Internacional ACROBAT, Romero, O.J., M. Orozco-Santos, R. Zapata-Altaminaro, A. Morfin-Valencia and J.A. Hernandez-Bautista, (Eds.). ACROBAT Publisher, Oaxaca, Mexico, pp: 265-267.
- Wong, W.C., 1986. In vitro propagation of banana (Musa sp.): Iitiation, proliferation and development of shoot-tip cultures on defined media. Plant Cell Tissue Organ Cult., 6: 159-166.
Direct Link - Zaid, A., 1987. In vitro browning of tissues and media with special emphasis to date palm cultures: A review. Acta Hortic., 212: 561-566.
CrossRefDirect Link