Submit Manuscript

Pakistan Journal of Biological Sciences

Year: 2007 | Volume: 10 | Issue: 21 | Page No.: 3895-3899
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail
Research Article

Comparison of Antioxidant and Free Radical Scavenging Activities of the Essential Oils from Flowers and Fruits of Otostegia persica Boiss

Fariba Sharififar, Valiolah Mozaffarian and Shirin Moradkhani

ABSTRACT

The aerial parts of the endemic plant of Otostegia persica Boiss. in two different stages of flowering and fruiting were hydro-distilled to extract oils in the yields of 0.3 and 0.15% (v/w), respectively. The oils were analyzed by GC and GC/MS. Twenty-eight and thirty-one components were identified, representing 97.59 and 94.61% of the oils, respectively. The main compounds of the essential oil flowers (EOFL) were alpha-pinene (17.21%), 1-octen,3-ol (13.44%) and cubenol (7.27%), whereas diisooctyl phthalate (45%) and hexadecanoic acid (11.07%) were the major constituents of the essential oil of the fruits (EOFR). The oils were screened for their possible antioxidant activities by two complementary test systems, namely DPPH free radical-scavenging and ammonium thiocyanate. In both tested systems, EOFL exerted greater antioxidant and radical scavenging activity. In the first case, EOFL exerted antioxidant activity with an IC50 19.8±1.8 μg mL-1 almost similar to BHA and ascorbic acid (15.2±1.1 and 17.4±1.3), respectively. In the ammonium thiocyanate system, the inhibition rate of oxidation of linoleic acid for EOFL was estimated 93.5±2.8. The higher activity of this oil in comparison to EOFR may be attributed to its high content of monoterpenes, especially oxygenated ones in the oil of the flowers.
PDF References Citation

Search

REFERENCES

  1. Adams, R.P., 1996. Identification of Essential Oil Components by Gas Chromatography/Quadrupole Mass Spectroscopy. Allured Publishing Corpoartion, Carol Stream, IL.
  2. Al-Dabbas, M.M., T. Suganuma, K. Kitahara, D.X. Hou and M. Fujii, 2006. Cytotoxic, antioxidant and antibacterial activities of Varthemia iphionoides Boiss. extracts. J. Ethnopharmacol., 108: 287-293.
    CrossRefDirect Link
  3. Aruoma, O.I., 2003. Methodological considerations for characterizing potential antioxidant actions of bioactive components in plant foods. Mutat. Res./Fundam. Mol. Mech. Mutagen., 523-524: 9-20.
    CrossRefDirect Link
  4. Burits, M. and F. Bucar, 2000. Antioxidant activity of Nigella sativa essential oil. Phytother. Res., 14: 323-328.
    CrossRefPubMedDirect Link
  5. Dudai, N., E. Putievsky, U. Ravid, D. Palevitch and A.H. Halevy, 1992. Monoterpene content in Origanum syriacum as effected by environmental conditions and flowering. Phys. Plant, 84: 453-459.
  6. Hajhashemi, V., M. Rabbani, G.R. Asghari and Z. Karami-Saravi, 2004. Effects of Otostegia persica (Burm) boiss on morphine withdrawal syndrome in mice. Iranian J. Pharmaceut. Res., 3: 30-35.
  7. Masude, T., J. Isobe, A. Jitoe and N. Nakamati, 1992. Antioxidant curcuminoids from rhizomes of Curcuma zanthorrhiza. Phytochemistry, 31: 3645-3647.
  8. Mata, A.T., C. Proenca, A.R. Ferreira, M.L.M. Serralheiro, J.M.F. Nogueira and M.E.M. Araujo, 2007. Antioxidant and antiacetylcholinesterase activities of five plants used as Portuguese food spices. Food Chem., 103: 778-786.
    CrossRefDirect Link
  9. Mozafarrian, V., 1998. Dictionary of the Names of Iranian Plants. Farhange Moaser Publisher, Tehran.
  10. Ormancey, X., S. Sisalli and P. Coutiere, 2001. Formulation of essential oils in functional parfumery. Parfums Cosmetiques Actualites, 157: 30-40.
  11. Ruberto, G. and M.T. Baratta, 2000. Antioxidant activity of selected essential oil components in two lipid model systems. Food Chem., 69: 167-174.
    CrossRefDirect Link
  12. Sacchetti, G., S. Maietti, M. Muzzoli, M. Scaglianti, S. Manfredini, M. Radice and R. Bruni, 2005. Comparative evaluation of 11 essential oils of different origin as functional antioxidants, antiradicals and antimicrobials in foods. Food Chem., 91: 621-632.
    CrossRefDirect Link
  13. Sawamura, M., 2000. Aroma and functional properties of Japaneseyuzu (Citrus junos Tanaka) essential oil. Aromatic Res., 1: 10-19.
  14. Scalbert, A., C. Manach, C. Morand and C. Remesy, 2005. Dietary of polyphenols and the prevention of diseases. Crit. Rev. Food Sci. Nutr., 45: 287-306.
    Direct Link
  15. Sharififar, F., N. Yassa and A. Shafiee, 2005. Antioxidant activity of Otostegia persica, (labiateae and its constituents. Iranian J. Pharmaceut. Res., pp: 235-239.
  16. Tepe, B., D. Daferera, A. Sokmen, M. Sokmen and M. Polissiou, 2005. Antimicrobial and antioxidant activities of the essential oil and various extracts of Salvia tomentosa Miller (Lamiaceae). Food Chem., 90: 333-340.
    CrossRefDirect Link
  17. Trouillas, P., C.A. Calliste, D.P. Allais, A. Simon, A. Marfak, C. Delage and J.L. Duroux, 2003. Antioxidant, anti-inflammatory and antiproliferative properties of sixteen water plant extracts used in the limousine countryside as herbal teas. Food Chem., 80: 399-407.
    CrossRef
  18. Yassa, N., F. Sharififar and A. Shafiee, 2005. Otostegia persica as a source of natural antioxidants. Pharm. Biol., 43: 33-38.
    CrossRef
  19. Zargari, A., 1989. Medicinal Plants. University of Tehran Press, Tehran.

Search

Related Articles

Leave a Comment

Your email address will not be published. Required fields are marked *