Harjono
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S. M. Widyastuti
Not Available
ABSTRACT
Purified 32-kDa endochitinase from Trichoderma reesei isolate T13 was tested for its antifungal activity in bioassay against the hyphal growth of soil-borne pathogenic fungus Ganoderma philippii. The results indicated that the degree of inhibition was proportional to the level of enzyme concentration applied. Inhibition zone visually occurred at the outer line of colony of G. philippii at 80 g ml-1 concentration of enzyme and above. The enzyme caused necrotic lesion on mycelial cell wall and stimulated branching response of mycelial tip at the concentration of 60 to 200 g ml-1. At the concentration of 100 to 200?g ml-1, endochitinase lyzed the hyphae of pathogenic fungus.
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How to cite this article
Harjono and S. M. Widyastuti, 2001. Antifungal Activity of Purified Endochitinase Produced by Biocontrol Agent Trichoderma reesei Against Ganoderma philippii. Pakistan Journal of Biological Sciences, 4: 1232-1234.
DOI: 10.3923/pjbs.2001.1232.1234
URL: https://scialert.net/abstract/?doi=pjbs.2001.1232.1234
DOI: 10.3923/pjbs.2001.1232.1234
URL: https://scialert.net/abstract/?doi=pjbs.2001.1232.1234
REFERENCES
- Hartree, E.F., 1972. Determination of protein: A modification of the lowry method that gives a linear photometric response. Anal. Biochem., 48: 422-427.
CrossRefPubMedDirect Link - Lorito, M., C.K. Hayes, A. Di Pietro, S.L. Woo and G.E. Harman, 1994. Purification, characterization and synergistic activity of a glucan 1,3 β-glucosidase and N-acetyl-β glucosaminidase from Trichoderma harzianum. Phytopathology, 84: 394-405.
Direct Link - Lorito, M., S.L. Woo, M. D'Ambrosio, G.E. Harman, C.K. Hayes, C.P. Kubicek and F. Scala, 1996. Synergistic interaction between cell wall degrading enzymes and membrane affecting compounds. Mol. Plant Microbe Interact., 9: 206-213.
Direct Link - Mauch, F., B. Mauch-Mani and T. Boller, 1988. Antifungal hydrolases in pea tissue: II. Inhibition of fungal growth by combinations of chitinase and β-1,3-glucanase. Plant Physiol., 88: 936-942.
CrossRefPubMedDirect Link - Sahai, A.S. and S.M. Manocha, 1993. Chitinases of fungi and plants: Their involvement in morphogenesis and host-parasite interaction. FEMS Microbiol. Rev., 11: 317-338.
CrossRefDirect Link - Schlumbaum, A., F. Mauch, U. Vogeli and T. Boller, 1986. Plant chitinases are potent inhibitors of fungal growth. Nature, 324: 365-367.
CrossRefDirect Link - Widyastuti, S.M., S. Sumardi and P. Sumantoro, 2001. Efektivitas Trichoderma spp. sebagai pengendali hayati terhadap tiga patogen tular tanah pada beberapa jenis tanaman kehutanan. (The effectiveness of Trichoderma spp. as biocontrol agents against three soil-borne pathogens of several forest tree spesies. Indon. J. Plant Protect., 7: 98-107.
- Widyastuti, S.M., S. Sumardi, I. Irfa and H.H. Nurjanto, 2002. Aktivitas penghambatan Trichoderma spp. formulasi terhadap jamur patogen tular tanah secara in vitro (In vitro inhibition activity of formulated Trichoderma spp. against soil-borne pathogenic fungi). J. Perlindungan Tanaman Indonesia, 8: 27-34.
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