A.A. Wasif
National center of Excellence in Molecular Biology, University of the Punjab, Canal Bank Road, Tokhar Niaz Baig, Lahore-53700. Pakistan
Haris
National center of Excellence in Molecular Biology, University of the Punjab, Canal Bank Road, Tokhar Niaz Baig, Lahore-53700. Pakistan
Samina Noor
National center of Excellence in Molecular Biology, University of the Punjab, Canal Bank Road, Tokhar Niaz Baig, Lahore-53700. Pakistan
Tayyab Hussain
National center of Excellence in Molecular Biology, University of the Punjab, Canal Bank Road, Tokhar Niaz Baig, Lahore-53700. Pakistan
S. Riazuddin
National center of Excellence in Molecular Biology, University of the Punjab, Canal Bank Road, Tokhar Niaz Baig, Lahore-53700. Pakistan
ABSTRACT
Zygotic embryos of cotton variety MNH-93 (Gossypium hirstum L.) were bombarded with tungsten particles coated with plasmid pActin 1-D carrying gene encoding β-glucuronidase (GUS). A helium pressure of 60 kg/cm, vacuun of 28 inch of Hg, target distance 22 cm, DNA and tungsten ratio0.50 μg: 240 μg and one-time shot showed maximum transformation efficiency by expressing a high detectable β-glucuronidase activity of GUS gene. This study will help in developing a protocol for the transformation of cotton varieties with agronomically important genes.
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How to cite this article
A.A. Wasif, Haris, Samina Noor, Tayyab Hussain and S. Riazuddin, 1999. Optimization of Parameters for the Transfer of Foreign Gene to Cotton (Gossypium hirsutum L.) by Particle Bombardment. Pakistan Journal of Biological Sciences, 2: 804-806.
DOI: 10.3923/pjbs.1999.804.806
URL: https://scialert.net/abstract/?doi=pjbs.1999.804.806
DOI: 10.3923/pjbs.1999.804.806
URL: https://scialert.net/abstract/?doi=pjbs.1999.804.806
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