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Residues of Tetracycline, Chloramphenicol and Tylosin Antibiotics in the Egyptian Bee Honeys Collected from Different Governorates



Asmaa E. Abd Alla
 
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ABSTRACT

Background and Objective: Bee honey as one of the most complete natural food for humans due to its therapeutic effect. Antibiotic residues in bee honey are still a significant problem in a wide range of the world. This study was conducted to determine three antibiotics residues, tylosin, chloramphenicol and tetracycline in the Egyptian bee honeys to give short brief about the Egyptian honeys status. Materials and Methods: Sixty-four bee honey samples (52 bee honey samples produced in different seasons and regions +12 samples from Egyptian supermarket) were collected from different types and governorates to determine the antibiotics residues by using high performance liquid chromatography. Results: Egyptian bee honey samples had contaminated by residues of 89 tylosin, 47% chloramphenicol and 31% tetracycline. Although, commonly used the antibiotics tylosin in the most tested samples, chloramphenicol recorded highest estimated residue. Conclusion: The chloramphenicol residues recorded the highest mean value comparing with tylosin and tetracycline residues in all types of bee honey except citrus and banana bee honeys. Natural bee honey should be free from any antibiotic residues and its presence due to wrong practices and lack of awareness of beekeepers, which negatively affected on human nutrition and health. Moreover, need to encourage beekeepers and urge them to transfer their colonies to newly reclaimed areas.

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  How to cite this article:

Asmaa E. Abd Alla , 2020. Residues of Tetracycline, Chloramphenicol and Tylosin Antibiotics in the Egyptian Bee Honeys Collected from Different Governorates. Pakistan Journal of Biological Sciences, 23: 385-390.

DOI: 10.3923/pjbs.2020.385.390

URL: https://scialert.net/abstract/?doi=pjbs.2020.385.390
 
Copyright: © 2020. This is an open access article distributed under the terms of the creative commons attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.

INTRODUCTION

Bee honey is the most important colony product of honey bees (Apis mellifera). It is the natural sweet substance produced by honey bees from the nectars of plant flowers and honey dew1. The bee honey has been considered as nutritive and therapeutic substances because it provided energy (80-85% carbohydrate) and monosaccharides which are easily digestible as those in many fruits2-4. In addition to many important substances such as organic acids, phenolyde compounds, flavonoids, flavonoids, antioxidants, pigments, vitamins, enzymes, etc5. The bee honey compositions depend on many factors such as geographical, floral origin, season, environmental and practices of beekeepers6,7.

Currently, bee honeys are produced in a polluted environment with many pollutant means (pesticides, heavy metals, antibiotics, bacteria and radioactivity) whether environmental or specific practice of beekeepers8,9. The honey contaminated from the beekeepers' erroneous practices is due to use of varroa parasites control and insecticides to control wax worms. Diseases and pests can be infected honey bee colonies, such as American, European foulbrood and nosemosis8. Sometimes antibiotics are used as a precaution to prevent infection10,11. Beekeepers are using tetracyclines, streptomycin, sulfonamides, tylosin, nitrofurans and chloramphenicol antibiotics for controlling diseases12,13. Additionally, these antibiotics are highly harmful for human health even it recorded with trace level in honey14. Antibiotic residues have a relatively long half-life period, which may have effects on consumers are directly toxic, allergies, may result in resistance to antibiotics in humans and damage to the central nervous system. Also, some antibiotics, such as chloramphenicol, which is known to cause tissue destruction, blood anemia, nitrofuran and possibly carcinogenic or causing fetal mutagenic15-17. Resistance of antibiotic is one of the 3 greatest threats to human health18, especially, a long-term exposure to antibiotics in humans, livestock, horticulture and food preservation. Oxytetracycline and chloramphenicol residues have been found above the regulatory standards in honey. Thus, determination of antibiotic residues in the bee honey was rigorous and should be free from organic or inorganic strange materials19-21.

In some EU countries, using antibiotics are illegal. There are no maximum residue limits established for antibiotics in honey according to European community regulations, this mean that honey contained antibiotics residues do not allowed for sale22-24. According to the previous studies, it is important to determine the antibiotics residues in the Egyptian bee honeys. Therefore, this current study aimed to determine residues of the most common three antibiotics (tetracycline, chloramphenicol and tylosin) in the Egyptian bee honeys.

MATERIALS AND METHODS

This investigation was carried out in the laboratory of the apiary yard, Experimental Station, Faculty of Agriculture, Cairo University at Giza. During 2017 year.

Honey samples: Sixty-four samples of Egyptian bee honey were collected from different Egyptian governorates as shown in Table 1.

Chemical analysis: Determinations of antibiotics (Tylosin, chloramphenicol and tetracycline) residues in each source of bee honey were done at food safety and quality control lap. Fac. Agric., Cairo Univ., Giza, Egypt. Using high performance liquid chromatography (HPLC), bee honey samples were analyzed according to Manual of Methods of Analysis of Food, Antibiotic and hormones residue25.

Statistical analysis: Data were analyzed using SAS software (SAS26). One-way analysis of variance (ANOVA) with unequal number of replications (Table 1) was used to compare among residues of three antibiotics within each types of honey and to compare among 7 different sources of honey within each antibiotic. When significant differences at p<0.05 were noted, Duncan's27 multiple range test was used to separate means.

RESULTS

Differences among sources of bee honey in antibiotics residues: Results in Table 2 showed that significant difference (p = 0.0058) among all sources of Egyptian bee honeys in its content of chloramphenicol antibiotics residue.

Table 1:
Sources of bee honey, number of samples and Egyptian governorates
All collected samples were stored at 20±2°C in the laboratory until the chemical analysis

Table 2:
Mean and range of three antibiotic residues in different sources of Egyptian bee honeys
a,b,x,yMeans followed by the same letters are not significantly different (p<0.05)

The significant higher values of chloramphenicol residue were appeared in market samples (16.06417±4.992 mg kg1) and ranged (0.0-59.2 mg kg1) compared to all the other different sources of bee honey samples followed by medical plants and cotton bee honey samples with no significant differences (13.75111±5.809 and 12.42125±5.78200 mg kg1) and (0.0-54.90 and 0.0-44.00 mg kg1) ranges, respectively.

The lowest values of chloramphenicol residue were observed in samples of sugar feeding honey (1.32±0.69 mg kg1 with range of 0.0-5.50 mg kg1) and clover bee honey (2.0967±0.775 mg kg1 and range of 0.0-9.80 mg kg1) with no significant differences. Moreover, no chloramphenicol residue values were shown in citrus and banana bee honeys.

Also results presented in Table 2 revealed no significant differences among all types of Egyptian bee honeys contaminated with tylosin (p = 0.2985) or tetracycline (p = 0.2092) residues.

Differences among antibiotics residues: Concerning the difference among antibiotics residues in bee honey, results in Table 2 show that grand mean of chloramphenicol residue was significantly the highest (6.521±2.7160 mg kg1) with range (0.0-54.90 mg kg1). However, the differences between residues of tylosin and tetracycline antibiotics were the lowest (0.0619±0.0142 and 0.002±0.0008 mg kg1 and ranged (0.0-0.242 mg kg1) and ranged between 0.0-0.066 mg kg1, respectively) with no significant differences as affected by sources of bee honey.

Practice of beekeepers: Figure 1 showed that the percentage of the Egyptian bee honeys containing one, two or three kinds of antibiotic residues reached 89, 52 or 13%, respectively. This may be due to the wrong practice of beekeepers in treating colony by antibiotics. Beekeepers usually use high doses for treating infection of bacterial brood and low doses to prevent infection. The obtained results are not accepted by The Quality Control of Egyptian Honey Specifications28 which states the bee honeys must not contain any antibiotics residue.

Fig. 1:
Percentage of bee honey samples that contaminated with 1, 2 or 3 antibiotic residues

Fig. 2:
Percentage of each antibiotic residue in the Egyptian bee honeys

Antibiotics residues survey: Results of the antibiotics residues survey which were appeared in different sources of bee honey samples are illustrated in Fig. 2. The highest contamination percentage (89%) for bee honey samples was with tylosin residue during different seasons. Except 11% of honey samples were not contaminated with the tylosin residue. The chloramphenicol residue was detected at 47% of bee honeys samples during different seasons and not detected in 53% samples. On the other hand, 31% of bee honey samples were contaminated with tetracycline residue but 69% of honey samples were not contaminated.

In general, 89% of different bee honey samples collected from different Egyptian governorates was contained with one antibiotic residue at least but 11%were free from any antibiotics residue. Tylosin antibiotic was the most commonly used followed by chloramphenicol and tetracycline. However, the values of chloramphenicol residues were the highest.

DISCUSSION

This study was investigated to determinate the three common antibiotic residues (tylosin, chloramphenicol and tetracycline) in the Egyptian bee honeys. Results indicated that from all samples 89% were contained with one antibiotic residue, 52% were contaminated with two kinds of antibiotics and 13% were contaminated with the three kinds. The bee honeys should be free from any antibiotic residues, presence of these substances, even in small quantities which affecting on marketing according to the quality control of Egyptian bee honey specifications28 and European Commission (EC) Directive24 with annexes states. The chloramphenicol residues were the highest values comparing with tylosin and tetracycline residues in all sources of bee honey except citrus and banana honeys. This is may be due to these honeys are produced in newly reclaimed land, which they depend on wells water as they are characterized by low relative humidity in this region. Consequently, these factors lead to reduce the spread of diseases and antibiotics in reclaimed land. So, we should encourage beekeepers and urge them to transfer their colonies to newly reclaimed areas. Furthermore, antibiotic residues in honey have become a major concern for consumers19. The reason of antibiotic residues presence in bee honeys is wrong practices of beekeepers not from the environment. Residue of antibiotics have a relatively long life span causing direct toxic effects on consumers such as allergic reactions to individuals with hypersensitivity and blood clotting disorder or indirect effect by stimulating bacterial strains to resistance15. Similar trends were observed by Payne et al.29 and Reybroeck30 whom reported that repeated exposure of bee honey antibiotic residues had toxic effect on blood, liver and bone marrow in human.

In Switzerland from 75 commercial honey samples, 34 samples were contaminated with originated materials31. Another study in Greece using HPLC analysis of 251 bee honey samples, 29% of the samples had antibiotic derived residues contamination. Most of them contained from 0.018-0.055 mg kg1 tetracycline residues and some others derivatives32 exceed 0.100 mg kg1.

In correspondence and parallel study, Sunay33 recorded that 25% of the samples contained sulfonamide and tetracycline group of antibiotics. Hammel et al.34 reported that in a limited survey of bee honey collected from different geographic origins showed that positive honey samples were often contaminated with more than one category of medicine .

In Switzerland, Ortelli et al.35 obtained that 75 commercially honey samples were tested, 13 samples (17%) including chloramphenicol residues from 0.4-6.0, 6 samples from 0.8-0.9 mg kg1 and 2 samples 5mg kg1. In Iran, a small amount of tylosin residues (0.3±0.1 ng g1) were detected in summer samples36. Barrasso et al.37 revealed that among 66 Apulian honey analyzed, 40% was detected by antibiotics (39 samples tylosin and 36 samples tetracycline residues). Finally, Antibiotics are not a component of bee honey but are the result of the misbehavior of beekeepers and the desire to maximize financial benefits without being aware of the effects on consumers. Beekeepers should be constantly aware of the adverse effects of the wrong practices in collecting, storing and trading the bee honey. Be careful when using antibiotics and use in the minimum, excluding the colonies that are treated from harvesting and collecting honey. Continuous encouragement to produce clean, healthy bee honey.

CONCLUSION

It concluded that under the Egyptian condition no significant differences in the Egyptian bee honeys contaminated with tylosin and tetracycline residues. However, a significant difference attributed to chloramphenicol residue contamination. This is referring to the use of beekeepers in large doses for the treatment of diseases without examining the effects on the presence of residuals on the health of consumers. Therefore, we should be monitoring the residues of antibiotic by beekeepers awareness to produce a healthy bee honey foods that free form antibiotics derivatives.

ACKNOWLEDGMENT

The author is gratefully acknowledged to late Prof. Dr. Mahmoud El- Sayed Nour Professor of beekeeping and economic insects, Fac. Agric., Cairo Univ. for investigation the idea of this search. The author also extends her sincere thanks and appreciation to Dr. Islam I. Omara professor of animal Production, Fac. Agric., Cairo Univ. for reviewing this search.

SIGNIFICANCE STATEMENT

This study discovers there are residues of antibiotics in bee honey that can be dependent on one kind of antibiotic and it's possible more than one kind together. That can be beneficial for reserve later from the use of antibiotics in the honeybee colony which honey is harvested. This study will help the researcher to uncover the critical areas in sources of contaminations and cheating of honey that many researchers were not able to explore. Thus a new theory on How to get bee honey without contamination? May be arrived at.

REFERENCES
Al-Waili, N., K. Salom, A. Al-Ghamdi and M.J. Ansari, 2012. Antibiotic, pesticide and microbial contaminants of honey: Human health hazards. Scient. World J., Vol. 2012. 10.1100/2012/930849

Alvarez-Suarez, J.M., F. Giampieri and M. Battino, 2013. Honey as a source of dietary antioxidants: Structures, bioavailability and evidence of protective effects against human chronic diseases. Curr. Med.Chem., 20: 621-638.
CrossRef  |  Direct Link  |  

Andreu, V., C. Blasco and Y. Pico, 2007. Analytical strategies to determine quinolone residues in food and the environment. Trends Anal. Chem., 26: 534-556.
CrossRef  |  Direct Link  |  

Barrasso, R., E. Bonerba, A. Savarino, E. Ceci, G. Bozzo and G. Tantillo, 2019. Simultaneous quantitative detection of six families of antibiotics in honey using a biochip multi-array technology. Vet. Sci., Vol. 6, No. 1. 10.3390/vetsci6010001

Bogdanov, S., 2006. Contaminants of bee products. Apidologie, 37: 1-18.
CrossRef  |  Direct Link  |  

Bogdanov, S., 2009. Honey control. Bee Product Science, M├╝hlethurnen, Switzerland. http://www.bee-hexagon.net/.

Bogdanov, S., T. Jurendic, R. Sieber and P. Gallmann, 2008. Honey for nutrition and health: A review. J. Am. Coll. Nutr., 27: 677-689.
CrossRef  |  Direct Link  |  

Chiesa, L.M., S. Panseri, M. Nobile, F. Ceriani and F. Arioli, 2018. Distribution of POPs, pesticides and antibiotic residues in organic honeys from different production areas. Food Addit. Contam.: Part A, 35: 1340-1355.
CrossRef  |  Direct Link  |  

Codex Alimentarius Commission, 2001. Draft revised standard for honey. World Health Organization and Food and Agriculture Organization, Rome, Italy. http://www.fao.org/3/X4616E/x4616e0b.htm.

Codex Alimentarius Commission, 2008. Veterinary drugs residues in food maximum residue limits. World Health Organization and Food and Agriculture Organization, Rome, Italy.

De Graaf, D.C., A.M. Alippi, K. Antunez, K.A. Aronstein and G. Budge et al., 2013. Standard methods for American foulbrood research. J. Apicult. Res., 52: 1-28.
CrossRef  |  Direct Link  |  

Duncan, D.B., 1955. Multiple range and multiple F tests. Biometrics, 11: 1-42.
CrossRef  |  Direct Link  |  

EOS., 2005. Bee honey and methods of analysis, Part 1. Egyptian Organization for Standardization and Quality Control, Cairo, Egypt, pp: 1-10.

El-Metwally, A.A.E., 2015. Factors affecting the physical and chemical characteristics of Egyptian beehoney. Ph.D. Thesis, Faculty of Agriculture, Cairo University, Egypt.

European Commission, 1990. Council Regulation (EEC) No 2377/90 of 26 June 1990 laying down a community procedure for the establishment of maximum residue limits of veterinary medicinal products in foodstuffs of animal origin. Official J. Eur. Communit., 224: 1-8.
Direct Link  |  

European Commission, 2002. Council Directive 2001/110/EC of 20 December 2001 relating to honey. Official J. Eur. Communit., 10: 47-52.
Direct Link  |  

European Commission, 2014. Directive 2014/63/EU of the European Parliament and of the council of 15 May 2014 amending Council Directive 2001/110/EC relating to honey. Official J. Eur. Communit., 164: 1-5.
Direct Link  |  

FSSAI., 2012. Manual of methods of analysis of foods: Antibiotics and hormones residues. Lab Manual 15, Food Safety and Standards Authority of India (FSSAI), Ministry of Health and Family Welfare, Government of India, pp: 1-54.

Forsgren, E., 2010. European foulbrood in honey bees. J. Invertebr. Pathol., 103: S5-S9.
CrossRef  |  Direct Link  |  

Genersch, E., 2010. American Foulbrood in honeybees and its causative agent, Paenibacillus larvae. J. Invertebr. Pathol., 103: S10-S19.
CrossRef  |  Direct Link  |  

Hammel, Y.A., R. Mohamed, E. Gremaud, M.H. LeBreton and P.A. Guy, 2008. Multi-screening approach to monitor and quantify 42 antibiotic residues in honey by liquid chromatography-tandem mass spectrometry. J. Chromatogr. A, 1177: 58-76.
CrossRef  |  Direct Link  |  

Hansen, H. and C.J. Brodsgaard, 1999. American foulbrood: A review of its biology, diagnosis and control. Bee World, 80: 5-23.
CrossRef  |  Direct Link  |  

Mahmoudi, R., R. Norian and M. Pajohi-Alamoti, 2014. Antibiotic residues in Iranian honey by ELISA. Int. J. Food Proper., 17: 2367-2373.
CrossRef  |  Direct Link  |  

Ortelli, D., P. Edder and C. Corvi, 2004. Analysis of chloramphenicol residues in honey by liquid chromatography-tandem mass spectrometry. Chromatographia, 59: 61-64.
CrossRef  |  Direct Link  |  

Payne, M., R.E. Baynes, S.E. Sundlof, A. Craigmill, A.I. Webb and J.E. Riviere, 1999. Drugs prohibited from extralabel use in food animals. J. Am. Vet. Med. Assoc., 215: 28-32.
Direct Link  |  

Rahman, M.M., A. Richardson and M. Sofian-Azirun, 2010. Antibacterial activity of propolis and honey against Staphylococcus aureus and Escherichia coli. Afr. J. Microbiol. Res., 4: 1872-1878.
Direct Link  |  

Reybroeck, W., 2003. Residues of antibiotics and sulphonamides in honey on the Belgian market. Apiacta, 38: 23-30.
Direct Link  |  

Reybroeck, W., 2014. Quality Control of Honey and Bee Products. In: Beekeeping for Poverty Alleviation and Livelihood Security, Volume 1: Technological Aspects of Beekeeping, Gupta, R.K., W. Reybroeck, J.W. van Veen and A. Gupta (Eds.). Chapter 18, Springer, Dordrecht, Netherlands, ISBN-13: 9789401791991, pp: 481-506.

Reybroeck, W., 2018. Residues of antibiotics and chemotherapeutics in honey. J. Apicult. Res., 57: 97-112.
CrossRef  |  Direct Link  |  

Reybroeck, W., E. Daeseleire, H.F. De Brabander and L. Herman, 2012. Antimicrobials in beekeeping. Vet. Microbiol., 158: 1-11.
CrossRef  |  Direct Link  |  

SAS., 2001. SAS User's Guide: Statistics. Release 8.02, SAS Institute Inc., Cary, NC., USA.

Saridaki-Papakonstadinou, M., S. Andredakis, A. Burriel and I. Tsachev, 2006. Determination of tetracycline residues in Greek honey. Trakia J. Sci., 4: 33-36.
Direct Link  |  

Sunay, A.E., 2006. Antibiotic residue problem in honey. Uludag Bee J., 4: 43-148.

Tillotson, G.S., G.V. Doern and J.M. Blondeau, 2006. Optimal antimicrobial therapy: The balance of potency and exposure. Expert Opin. Invest. Drugs, 15: 335-337.
CrossRef  |  Direct Link  |  

Van den Heever, J.P., T.S. Thompson, J.M. Curtis and S.F. Pernal, 2015. Determination of dicyclohexylamine and fumagillin in honey by LC-MS/MS. Food Anal. Methods, 8: 767-777.
CrossRef  |  Direct Link  |  

White, J.W. and L.W. Doner, 1980. Honey composition and properties. Agriculture Handbook No. 335, Beekeeping in the United States, pp: 82-91. http://www.beesource.com/resources/usda/honey-composition-and-properties/.

Zhang, Y., X.Q. Li, H.M. Li, Q.H. Zhang, Y. Gao and X.J. Li, 2019. Antibiotic residues in honey: A review on analytical methods by liquid chromatography tandem mass spectrometry. Trends Anal. Chem., 110: 344-356.
CrossRef  |  Direct Link  |  

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