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Research Article
 

Evaluation of Different Wheat Varieties by SDS-PAGE Electrophoresis



Mohd Shuaib , Ikhtiar Khan , Zahir Ali , Waqar Ali , Taufiq Ahmad and Alam Zeb
 
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ABSTRACT

Wheat seed-storage proteins represent not only an important source of food and energy but it is also involved in the determination of bread-making quality. Wheat grains of thirteen wheat varieties were collected from different ecological regions of Pakistan. The variability of seed storage-proteins was analyzed by using SDS-PAGE electrophoresis. Electrophorogram for each variety was scored and presence or absence of each band noted and entered in a binary data matrix. Based on electrophoresis band spectra, Jaccard‘s Similarity Index (JSI) was calculated. Genetic diversity of wheat was evaluated by constructing the dendrogram for High Molecular Weight (HMW) and Low Molecular Weight (LMW) gluten subunit bands. It is concluded that seed storage protein profiles could be useful markers in the studies of genetic diversity and classification of adapted cultivars, thereby improving the efficiency of wheat breeding programs in cultivar development especially in developing countries like Pakistan.

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  How to cite this article:

Mohd Shuaib , Ikhtiar Khan , Zahir Ali , Waqar Ali , Taufiq Ahmad and Alam Zeb , 2007. Evaluation of Different Wheat Varieties by SDS-PAGE Electrophoresis. Pakistan Journal of Biological Sciences, 10: 1667-1672.

DOI: 10.3923/pjbs.2007.1667.1672

URL: https://scialert.net/abstract/?doi=pjbs.2007.1667.1672

REFERENCES
1:  Bietz, J.A. and J.S. Wall, 1973. Isolation and characterization of gliadin-like subunits from glutenins. Cereal Chem., 50: 537-547.
Direct Link  |  

2:  Bietz, J.A. and J.S. Wall, 1972. Wheat gluten subunits: Molecular weights determined by sodium sulfate-polyacrylamide gel electrophoresis. Cereal Chem., 49: 416-430.

3:  Branlard, G., J.C. Autran and P. Monneveux, 1989. High molecular weight glutenin subunits in durum wheat (Triticum durum). Theor. Applied Genet., 78: 353-358.

4:  Cooke, R.J. and J.R. Law, 1998. Seed storage protein diversity in wheat varieties. Plant Varieties Seeds, 11: 159-167.

5:  Damania, A.B., E. Porceddu and M.T. Jackson, 1983. A rapid method for the evaluation of variation in germplasm collections of cereals using polyacrylamide gel electrophoresis. Euphytica, 32: 877-883.

6:  Fufa, H., P.S. Baenziger, B.S. Beecher, I. Dweikat, R.A. Graybosch and K.M. Eskridge, 2005. Comparison of phenotypic and molecular marker-based classifications of hard red winter wheat cultivars. Euphytica, 145: 133-146.
CrossRef  |  Direct Link  |  

7:  Khan, M.F., E. Schumann and W.E. Weber, 2002. Characterisation of Pakistani wheat varieties for general cultivation in the mountaineous regions of Azad Kashmir. Asian J. Plant Sci., 1: 699-702.
CrossRef  |  Direct Link  |  

8:  Lawrence, G.J. and K.W. Shepherd, 1980. Variation in glutenin protein subunits of wheat. Aust. J. Biol. Sci., 33: 221-233.
Direct Link  |  

9:  MacRitchie, F., 1994. Role of Polymeric Proteins in Flour Functionality in Wheat Kernel Proteins: Molecular and Functional Aspects. Universita Degli Studi Della Tuscia, Bitervo, Italy, pp: 145-150.

10:  Payne, P.I., C.N. Law and E.E. Mudd, 1980. Control by homoeologous group 1 chromosome of the high-molecular-weight subunits of glutenin, a major protein of wheat endosperm. Theor. Applied Genet., 58: 113-120.

11:  Payne, P.I., L.M. Holt and C.N. Law, 1981. Structural and genetical studies on the high-molecular-weight subunits of wheat glutenin. Part I: Alleic variation in subunits amongst varieties of wheat (T. aestivum). Theor. Applied Genet., 60: 229-236.
CrossRef  |  Direct Link  |  

12:  Payne, P.I., L.M. Holt, G.J. Lawrence and C.N. Law, 1982. The genetics of gliadin and glutenin, the major storage proteins of the wheat endosperm. Qual. Plant. Plant Foods Hum. Nutr., 31: 229-241.
CrossRef  |  Direct Link  |  

13:  Payne, P.I, L.M. Holt, E.A. Jackson and C.N. Law, 1984. Wheat storage proteins: Their genetics and their potential for manipulation by plant breeding. Phil. Trans. R. Soc. Lond., 304: 359-371.

14:  Rohlf, F.J., 1993. NTSYS-pc. Numerical Taxonomy and Multivariate Analysis System. Version 1.80, Applied Biostatistics Inc., New York.

15:  Sneath, P.H.A. and R.R. Sokal, 1973. Numerical Taxonomy: The Principle and Practice of Numerical Classification. W.F. Freeman and Co., San Francisco, pp: 573.

16:  Xueli, A., L. Qiaoyun, Y. Yueming, X. Yinghua, S.L.K. Hsam and F.J. Zeller, 2005. Genetic diversity of European spelt wheat (Triticum aestivum ssp. Spelta L. em. Thell.) revealed by glutenin subunit variations at the Glu-1 and Glu-3 loci. Euphytica, 146: 193-201.
Direct Link  |  

17:  Zeb, A., Z. Ali, T. Ahmad and A. Abdumanon, 2006. Physicochemical characteristics of wheat varieties growing in the same and different ecological regions of Pakistan. Pak. J. Biol. Sci., 9: 1823-1828.
CrossRef  |  Direct Link  |  

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