Abdulrahman Bahrami
Faculty of Health, Ramadan University of Medical Science, P.O. Box 4171-65175, Ramadan, Iran
John Edwards
Department of Environmental Health, School of Medicine,
Flinders University, Adelaide, South Australia, 5001
ABSTRACT
The objectives of this study were to develop a method for analysis of trans, trans-muconic acid (tt-MA) of urine in group of children living in Adelaide and compare to those residing in a rural zone in the South Australia. tt-MA was extracted from urine by ethyl acetate and analyzed with High Performance Liquid Chromatography (HPLC). The mean recovery of tt-MA from urine for spiked sample was 94 to 99%. There was a significant difference between tt-MA level for children living in Adelaide compare to children living in a rural at South Australia (p<0.05). Also a significant difference was found between tt-MA level for children living less than 100 m from road compare those living more than 100 m (p<0.05). The sensitive and specific HPLC analysis has been developed for the simultaneous measurement of tt-MA and tt-MA can be utilized as a biomarker for exposure to benzene in children.
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How to cite this article
Abdulrahman Bahrami and John Edwards, 2005. Determination of trans, trans-muconic acid in Children Living in Adelaide Based on HPLC Developed Method. Pakistan Journal of Biological Sciences, 8: 1703-1706.
DOI: 10.3923/pjbs.2005.1703.1706
URL: https://scialert.net/abstract/?doi=pjbs.2005.1703.1706
DOI: 10.3923/pjbs.2005.1703.1706
URL: https://scialert.net/abstract/?doi=pjbs.2005.1703.1706
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